Evaluating the Function of Melting Point Temperature of Phylogenetic Genes for Detecting the Taxons of Snakes

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Moradi Jaferi Leila; Kazemi Noureini Sakineh; RASTEGAR POUYANI ESKANDAR

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Journal: JOURNAL OF ANIMAL BIOLOGY Year:2019 | Volume:11 | Issue:3 Page(s): 71-84

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Title

Evaluating the Function of Melting Point Temperature of Phylogenetic Genes for Detecting the Taxons of Snakes

Author(s)

Moradi Jaferi Leila | Kazemi Noureini Sakineh | RASTEGAR POUYANI ESKANDAR | Issue Writer Certificate

Keywords

Melting temperatureQ1

Molecular phylogenyQ1

SnakesQ1

Cytochrom b geneQ1

16 S geneQ1

Abstract

Deoxyribonucleic acid (DNA) is the molecule encoding the genetic instructions used in the growth and functioning of all living organisms and is very appropriate for biological information storage. Todays, the DNA sequences are regarded as sources for detecting the species and their phylogenetic relationships. Despite the modern technologies including the next generation sequencing and DNA barcoding, there is still a need for a fast, cheap, and reliable method for identifying species particularly in ambiguous morphologically-defined organisms. This study evaluated to see if some biophysical chemistry properties of PCR products like melting point can be used before sequencing to identify the samples of interest. Determining the Melting temperature of PCR products is available in most available measurements in molecular biology labs. The present study focused on the potential use of Melting temperature related to PCR products of 16S and Cyt b (highly used genes in phylogenetic studies) using a real-time PCR machine to identify different species of Snakes from Iran. The preliminary results of these two genes are promising although extra steps are required to improve its sensitivity. The development of this method can be used in a rapid evaluation of species.

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APA: Copy

Moradi Jaferi, Leila, Kazemi Noureini, Sakineh, & RASTEGAR POUYANI, ESKANDAR. (2019). Evaluating the Function of Melting Point Temperature of Phylogenetic Genes for Detecting the Taxons of Snakes. JOURNAL OF ANIMAL BIOLOGY, 11(3 ), 71-84. SID. https://sid.ir/paper/176940/en

Vancouver: Copy

Moradi Jaferi Leila, Kazemi Noureini Sakineh, RASTEGAR POUYANI ESKANDAR. Evaluating the Function of Melting Point Temperature of Phylogenetic Genes for Detecting the Taxons of Snakes. JOURNAL OF ANIMAL BIOLOGY[Internet]. 2019;11(3 ):71-84. Available from: https://sid.ir/paper/176940/en

IEEE: Copy

Leila Moradi Jaferi, Sakineh Kazemi Noureini, and ESKANDAR RASTEGAR POUYANI, “Evaluating the Function of Melting Point Temperature of Phylogenetic Genes for Detecting the Taxons of Snakes,” JOURNAL OF ANIMAL BIOLOGY, vol. 11, no. 3 , pp. 71–84, 2019, [Online]. Available: https://sid.ir/paper/176940/en

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