IN VITRO ANTIMICROBIAL ACTIVITY OF METHANOL EXTRACT OF ORANGE (CITRUS SINENSIS) PEEL AGAINST HELICOBACTER PYLORI CLINICAL ISOLATES

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NAKHAEI MOGHADAM M.

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2009 | Volume:1 | Issue:2 (2) Page(s): 37-43

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Title

IN VITRO ANTIMICROBIAL ACTIVITY OF METHANOL EXTRACT OF ORANGE (CITRUS SINENSIS) PEEL AGAINST HELICOBACTER PYLORI CLINICAL ISOLATES

Author(s)

NAKHAEI MOGHADAM M. | Issue Writer Certificate

Keywords

HELICOBACTER PYLORIQ2

ORANGE PEELQ2

CITRUS SINENSISQ3

EXTRACTQ3

Abstract

Background and Objective: Although HELICOBACTER PYLORI infection could be eradicated by a combination of therapeutic agents, but sometimes the cure thus achieved is incomplete and undesirable side effects are certain to occur. ORANGE PEEL is used as a food additive for flavoring. Iranian people use the orange rind to improve flavor of cake or some foods such as rice. Considering the use of ORANGE PEEL for digestive disorders in traditional medicine and the increased development of resistance of HELICOBACTER PYLORI to antibiotics, we decided to evaluate the anti- HELICOBACTER PYLORI activity of ORANGE PEEL.Materials and Methods: Percolated methanol EXTRACT of ORANGE PEEL were tested against 37 clinical isolated of HELICOBACTER PYLORI. Growth inhibition was determined by the filter paper disc diffusion method on modified egg yolk emulsion agar compared with amoxicillin and metronidazole.Results: All of bacterial isolates were sensitive to 2 mg of ORANGE PEEL EXTRACT. The mean of inhibition zone was 13.28±0.45 mm. The minimum inhibitory concentration (MIC) of methanol EXTRACT was 729 mg/ml. MBC was more than MIC. Methanol EXTRACT preserved its anti- HELICOBACTER PYLORI activity after autoclaving for 20 minutes.Conclusion: Screening among natural resources and plants which are used in folk medicine for gastric problems can be benefit .This study demonstrated that ORANGE PEEL inhibits growth of HELICOBACTER PYLORI isolates in vitro. Concerning the other benefits of herbal medicines, using of ORANGE PEEL is recommended instead of vanillin to prepare foodstuffs such as cakes.

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APA: Copy

NAKHAEI MOGHADAM, M.. (2009). IN VITRO ANTIMICROBIAL ACTIVITY OF METHANOL EXTRACT OF ORANGE (CITRUS SINENSIS) PEEL AGAINST HELICOBACTER PYLORI CLINICAL ISOLATES. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 1(2 (2)), 37-43. SID. https://sid.ir/paper/181410/en

Vancouver: Copy

NAKHAEI MOGHADAM M.. IN VITRO ANTIMICROBIAL ACTIVITY OF METHANOL EXTRACT OF ORANGE (CITRUS SINENSIS) PEEL AGAINST HELICOBACTER PYLORI CLINICAL ISOLATES. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2009;1(2 (2)):37-43. Available from: https://sid.ir/paper/181410/en

IEEE: Copy

M. NAKHAEI MOGHADAM, “IN VITRO ANTIMICROBIAL ACTIVITY OF METHANOL EXTRACT OF ORANGE (CITRUS SINENSIS) PEEL AGAINST HELICOBACTER PYLORI CLINICAL ISOLATES,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 1, no. 2 (2), pp. 37–43, 2009, [Online]. Available: https://sid.ir/paper/181410/en

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