ISOLATION OF OLEAGINOUS YEASTS AND OPTIMIZATION OF LIPID PRODUCTION USING TAGUCHI DESIGN

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ABDOLI A.; ENSHAEIEH M.; NAHVI I.; MADANI M.

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2012 | Volume:4 | Issue:14 Page(s): 13-20

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Title

ISOLATION OF OLEAGINOUS YEASTS AND OPTIMIZATION OF LIPID PRODUCTION USING TAGUCHI DESIGN

Author(s)

ABDOLI A. | ENSHAEIEH M. | NAHVI I. | MADANI M. | Issue Writer Certificate

Keywords

OLEAGINOUS YEASTSQ2

OPTIMIZATIONQ2

TRI ACYL GLYCEROLQ2

Abstract

Background and Objective: Micro-organisms that accumulate lipid more than 20% of their biomass are introduced as oleaginous strains. Oleaginous yeast accumulates TRI ACYL GLYCEROL rich in unsaturated fatty acids. These lipophylic microbial compounds, because of their special characteristics, are considered from industrial point of view. Microbial oil has potential for biodiesel production. Biodiesel compared to fossil fuel is very peaceful to the environment.Materials and Methods: In this investigation 70 yeast strains were isolated from soil of different regions and 23 of them were oleaginous. Screening of oleaginous strains was done by enrichment in Glycerin medium and sudan black staining. Then the amount of lipid production was measured by Bligh & Dyer method. Among the isolates one of them with Aa2 ID was selected for identification and further investigations.Results: After OPTIMIZATION in medium containing 95 g/l Glucose, 1.5g/l (NH4)2SO4, 3g/l KH2PO4, 1.5 g/l MgSO4.7H2O & 2g/l NaH2PO4 and 1g/l yeast extract for 96h at 150 rpm with a pH of 5.5, the strain (Aa2) had lipid production, dry biomass & lipid quantity of 5.44g/L, 14.39 g/L & 37.8%.Conclusion: The results obtained in this study indicate that there are valuable native strains in our country that by isolation and OPTIMIZATION of lipid production in them, they can be used in different industrial cases such as biodiesel production without needing foreign strains.

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APA: Copy

ABDOLI, A., ENSHAEIEH, M., NAHVI, I., & MADANI, M.. (2012). ISOLATION OF OLEAGINOUS YEASTS AND OPTIMIZATION OF LIPID PRODUCTION USING TAGUCHI DESIGN. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 4(14), 13-20. SID. https://sid.ir/paper/181433/en

Vancouver: Copy

ABDOLI A., ENSHAEIEH M., NAHVI I., MADANI M.. ISOLATION OF OLEAGINOUS YEASTS AND OPTIMIZATION OF LIPID PRODUCTION USING TAGUCHI DESIGN. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2012;4(14):13-20. Available from: https://sid.ir/paper/181433/en

IEEE: Copy

A. ABDOLI, M. ENSHAEIEH, I. NAHVI, and M. MADANI, “ISOLATION OF OLEAGINOUS YEASTS AND OPTIMIZATION OF LIPID PRODUCTION USING TAGUCHI DESIGN,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 4, no. 14, pp. 13–20, 2012, [Online]. Available: https://sid.ir/paper/181433/en

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