EVALUATION OF HBV PREVALENCE IN HEMODIALYSIS PATIENT OF TEHRAN BY PCR

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SHAHHOSSEINY M.H.; DABAGHIZADEH A.; MOSLEMI E.; HAGH ZAREH M.R.; YAGHMAEE P.

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2011 | Volume:3 | Issue:8 Page(s): 37-42

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Title

EVALUATION OF HBV PREVALENCE IN HEMODIALYSIS PATIENT OF TEHRAN BY PCR

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Keywords

HEMODIALYSISQ2

PCRQ2

HEPATITIS B VIRUSQ2

Abstract

Background and Objective: HEPATITIS B VIRUS infection prevalence is more significant in HEMODIALYSIS patients than the others. Serological HBV detection techniques could not detect the infection in early stages. On the bases of common detection methods limitations, molecular detection techniques like PCR are more useful in HEMODIALYSIS patients. In this study it has tried to applied PCR technique for the detection of HBV Virus in HEMODIALYSIS patient's serums.Materials and Methods: 136 serum samples were obtained from patient who referred to three in HEMODIALYSIS centers in Tehran. DNA was extracted by using DNP kit. The sensitivity and specificity of test were performed and after that was done on samples. PCR product was electrophoresed in 1.5% agarose gel. Amplicon (262 bp) cloned by PCR-cloning and then sequenced by dideoxy chain termination.Results: PCR test had high specificity which no reaction was seems to the other infectious agents DNA except HEPATITIS B VIRUS DNA. The PCR sensitivity up to 40 particles was observed. Among the 136 serum samples 11 cases (8.09%) were PCR positive in our study, but only 1 case were ELISA positive.Conclusion: The compression between ELISA and PCR application on HEMODIALYSIS patients suggested that the employed assay system constitutes an effective tool for the rapid diagnosis of HEPATITIS B VIRUS especially in HEMODIALYSIS patients. In addition because of low serological techniques sensitivity for detection, it seems PCR could be an appropriate replacement for these detection techniques.

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APA: Copy

SHAHHOSSEINY, M.H., DABAGHIZADEH, A., MOSLEMI, E., HAGH ZAREH, M.R., & YAGHMAEE, P.. (2011). EVALUATION OF HBV PREVALENCE IN HEMODIALYSIS PATIENT OF TEHRAN BY PCR. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 3(8), 37-42. SID. https://sid.ir/paper/181442/en

Vancouver: Copy

SHAHHOSSEINY M.H., DABAGHIZADEH A., MOSLEMI E., HAGH ZAREH M.R., YAGHMAEE P.. EVALUATION OF HBV PREVALENCE IN HEMODIALYSIS PATIENT OF TEHRAN BY PCR. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2011;3(8):37-42. Available from: https://sid.ir/paper/181442/en

IEEE: Copy

M.H. SHAHHOSSEINY, A. DABAGHIZADEH, E. MOSLEMI, M.R. HAGH ZAREH, and P. YAGHMAEE, “EVALUATION OF HBV PREVALENCE IN HEMODIALYSIS PATIENT OF TEHRAN BY PCR,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 3, no. 8, pp. 37–42, 2011, [Online]. Available: https://sid.ir/paper/181442/en

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