LOOP MEDIATED ISOTHERMAL AMPLIFICATION OF HEPATITIS B VIRUS IN HEMODIALYSIS PATIENTS

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MOHMMAD HASAN SHAHHOSSEINY; ESMAILII ZOHRE; MOSLEMI ELHAM; YAGHMAII PARICHEHRE

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Journal: JOURNAL OF MICROBIAL WORLD Year:2009 | Volume:2 | Issue:3 Page(s): 183-191

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Information Journal Paper

Title

LOOP MEDIATED ISOTHERMAL AMPLIFICATION OF HEPATITIS B VIRUS IN HEMODIALYSIS PATIENTS

Author(s)

MOHMMAD HASAN SHAHHOSSEINY | ESMAILII ZOHRE | MOSLEMI ELHAM | YAGHMAII PARICHEHRE | Issue Writer Certificate

Keywords

HEPATITIS BQ2

HEMODIALYSISQ2

LAMPQ3

PCRQ2

Abstract

Background and Objectives: Infections with HEPATITIS B virus in HEMODIALYSIS centers still remain as a major problem. The propose of this study is optimization and development of LAMP technique for assessment of HBV infection in serum of HEMODIALYSIS patients.Material and Methods: This cross sectional study was performed on totally, 136 serum samples were obtained from the patients how referred to hemodyalis center in Tehran as: 52 serum samples from Mastafa khomaini hospital, 54 serum samples from Chamran hospital and 31 serum samples from Erfan hospital. After DNA extraction, 6 HBsAg specific primers were used for optimizing of reaction conditions and material concentration for LAMP. LAMP products were evaluated by adding 1% SYBER Green and electrophoresis stained by Ethidium-Bromide. Results: LAMP sensitivity was determined as 4 particles in this study. In addition, LAMP technique Showed a high specify for detecting the viruses. Totally, 13 and 7 cases of the serum samples of Mastafa khomaini were positive by LAMP and PCR respectfully. Also, 4 and 2 samples for second group and 2 and 2 samples for third group were positive respectfully by LAMP and PCR. Conclusion: The LAMP technique is a more sensitive and cost friendly system to detect HBV in comparison to conventional PCR.

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APA: Copy

MOHMMAD HASAN, SHAHHOSSEINY, ESMAILII, ZOHRE, MOSLEMI, ELHAM, & YAGHMAII, PARICHEHRE. (2009). LOOP MEDIATED ISOTHERMAL AMPLIFICATION OF HEPATITIS B VIRUS IN HEMODIALYSIS PATIENTS. JOURNAL OF MICROBIAL WORLD, 2(3), 183-191. SID. https://sid.ir/paper/189286/en

Vancouver: Copy

MOHMMAD HASAN SHAHHOSSEINY, ESMAILII ZOHRE, MOSLEMI ELHAM, YAGHMAII PARICHEHRE. LOOP MEDIATED ISOTHERMAL AMPLIFICATION OF HEPATITIS B VIRUS IN HEMODIALYSIS PATIENTS. JOURNAL OF MICROBIAL WORLD[Internet]. 2009;2(3):183-191. Available from: https://sid.ir/paper/189286/en

IEEE: Copy

SHAHHOSSEINY MOHMMAD HASAN, ZOHRE ESMAILII, ELHAM MOSLEMI, and PARICHEHRE YAGHMAII, “LOOP MEDIATED ISOTHERMAL AMPLIFICATION OF HEPATITIS B VIRUS IN HEMODIALYSIS PATIENTS,” JOURNAL OF MICROBIAL WORLD, vol. 2, no. 3, pp. 183–191, 2009, [Online]. Available: https://sid.ir/paper/189286/en

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