SEROGROUP SCREENING OF SALMONELLA ISOLATED FROM POULTRY AND DETECTION OF THEIR HILA GENE BY PCR ASSAY

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AKBARMEHR J.

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2010 | Volume:2 | Issue:6 Page(s): 33-38

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Title

SEROGROUP SCREENING OF SALMONELLA ISOLATED FROM POULTRY AND DETECTION OF THEIR HILA GENE BY PCR ASSAY

Author(s)

AKBARMEHR J. | Issue Writer Certificate

Keywords

PCRQ2

POULTRYQ2

SALMONELLAQ2

HILAQ3

Abstract

Background and Objective: Salmonellosis is an important infectious disease in both animals and humans. Food of POULTRY origin is the most important sources of SALMONELLA infection in humans.The hyperinvasive locus A (hilA) gene has an important role in SALMONELLA pathogenicity. This gene encodes a transcriptional regulator that activates the expression of invasion gene and facilitates the entry of the bacteria into intestinal epithelial cells. The goal of this research was to isolate SALMONELLA from POULTRY, serogroup screening of them and detect of their HILA gene by Polymerase Chain Reaction (PCR) technique.Material and Methods: This research which was carried out in East Azarbayjan province in Iran, overall 520 samples were obtained from chicken (400 samples), ostrich (30 samples), and pigeon (90 samples). Samples were harvested from intestine, liver, spleen of POULTRY and examined by bacteriological tests. Serogroup screening was done by specific SALMONELLA antisera. PCR was applied with one pair of primers targeting the SALMONELLA HILA gene.Results: According to the results of bacteriological tests 45 SALMONELLA strains were found among 520 of total samples (8.65%). The prevalence of SALMONELLA spp in chicken, ostrich, and pigeon were determined as7.25%, 6.66% and 15.55%, respectively. SALMONELLA isolates represented four different serogroups (D1, B, C1, and C2) which SALMONELLA serogroup D1 with frequency 53.3% was the most dominant serogroup. The PCR of the HILA gene produced a band of 854 bp with all the SALMONELLA isolates.Conclusion: This research was indicated the relatively high frequency of SALMONELLA serogroup D1 in POULTRY and the high prevalence of salmonellosis in pigeon and also suggested that the HILA gene could be present in different SALMONELLA serogroups from different hosts in birds.

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APA: Copy

AKBARMEHR, J.. (2010). SEROGROUP SCREENING OF SALMONELLA ISOLATED FROM POULTRY AND DETECTION OF THEIR HILA GENE BY PCR ASSAY. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 2(6), 33-38. SID. https://sid.ir/paper/181446/en

Vancouver: Copy

AKBARMEHR J.. SEROGROUP SCREENING OF SALMONELLA ISOLATED FROM POULTRY AND DETECTION OF THEIR HILA GENE BY PCR ASSAY. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2010;2(6):33-38. Available from: https://sid.ir/paper/181446/en

IEEE: Copy

J. AKBARMEHR, “SEROGROUP SCREENING OF SALMONELLA ISOLATED FROM POULTRY AND DETECTION OF THEIR HILA GENE BY PCR ASSAY,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 2, no. 6, pp. 33–38, 2010, [Online]. Available: https://sid.ir/paper/181446/en

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