EVALUATION OF SOIL-ISOLATED AND STANDARD STRAIN OF BACILLUS PUMILUS AGAINST TWO PHYTOPATHOGENS AND THREE HUMAN PATHOGENS AND OPTIMIZATION OF CONDITIONS OF ANTIMICROBIAL COMPOUNDS PRODUCTION

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POURBAGHI E.; EISAZADEH K.; ANVARI M.; MODIRI L.

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2012 | Volume:4 | Issue:13 Page(s): 45-52

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Title

EVALUATION OF SOIL-ISOLATED AND STANDARD STRAIN OF BACILLUS PUMILUS AGAINST TWO PHYTOPATHOGENS AND THREE HUMAN PATHOGENS AND OPTIMIZATION OF CONDITIONS OF ANTIMICROBIAL COMPOUNDS PRODUCTION

Author(s)

POURBAGHI E. | EISAZADEH K. | ANVARI M. | MODIRI L. | Issue Writer Certificate

Keywords

NATURAL ANTIMICROBIAL COMPOUNDSQ2

B.PUMILUSQ2

SCREENINGQ2

Abstract

Background and Objective: Soil-derived microorganisms have proven to be a useful source of NATURAL ANTIMICROBIAL COMPOUNDS. By antagonistic activity of B.PUMILUS species against some phytopathogens and antimicrobial activity against pathogenic bacteria and fungi, this study was designed with the aim of evaluation of antimicrobial activity of isolate and standard strain of B. pumilus against two phytopathogens P. carotovorum and Xanthomonas campestris and three human pathogens C.albicans, B.cereus and Ps. aeruginosa and optimization of conditions of antimicrobials production.Materials and Methods: For isolation of B.PUMILUS species serial dilution agar plate technique, biochemical tests and antibiotic susceptibility assay were used. For the initial study of antagonistic activity, trypticase soy broth medium and agar disk diffusion methods were used; and for production of antimicrobial compounds, synthetic medium and agar well diffusion method were used. Then, optimization of conditions of antimicrobial compounds production was evaluated by the change of parameters like pH (6-9), glucose concentration (1-5%) and incubation period (0-72 hours). Data were analyzed using SPSS and statistical tests of variance analysis (ANOVA).Results: Isolate and standard strain of B.PUMILUS showed better antimicrobial activity against Gram-positive human pathogen compared to other test microorganisms. Maximum production of antimicrobial compounds was obtained at pH 7, 3% glucose and after 48 hours of incubation at 37oC against target microorganisms. Also, a significant relationship between change of pH and glucose concentration of the culture media and incubation period for more production of antimicrobial compounds was observed (P<0.05).Conclusion: The results showed that type and compositions of used media and external factors like pH and incubation period are effective on more production of antimicrobial compounds of B. pumilus.

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APA: Copy

POURBAGHI, E., EISAZADEH, K., ANVARI, M., & MODIRI, L.. (2012). EVALUATION OF SOIL-ISOLATED AND STANDARD STRAIN OF BACILLUS PUMILUS AGAINST TWO PHYTOPATHOGENS AND THREE HUMAN PATHOGENS AND OPTIMIZATION OF CONDITIONS OF ANTIMICROBIAL COMPOUNDS PRODUCTION. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 4(13), 45-52. SID. https://sid.ir/paper/181461/en

Vancouver: Copy

POURBAGHI E., EISAZADEH K., ANVARI M., MODIRI L.. EVALUATION OF SOIL-ISOLATED AND STANDARD STRAIN OF BACILLUS PUMILUS AGAINST TWO PHYTOPATHOGENS AND THREE HUMAN PATHOGENS AND OPTIMIZATION OF CONDITIONS OF ANTIMICROBIAL COMPOUNDS PRODUCTION. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2012;4(13):45-52. Available from: https://sid.ir/paper/181461/en

IEEE: Copy

E. POURBAGHI, K. EISAZADEH, M. ANVARI, and L. MODIRI, “EVALUATION OF SOIL-ISOLATED AND STANDARD STRAIN OF BACILLUS PUMILUS AGAINST TWO PHYTOPATHOGENS AND THREE HUMAN PATHOGENS AND OPTIMIZATION OF CONDITIONS OF ANTIMICROBIAL COMPOUNDS PRODUCTION,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 4, no. 13, pp. 45–52, 2012, [Online]. Available: https://sid.ir/paper/181461/en

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