STUDY OF CRUDE OIL BIOREMEDIATION BY BACTERIUM ISOLATED FROM SOILS CONTAMINATED WITH PETROLEUM HYDROCARBONS

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EBRAHIMIPOUR GH.; KARKHANE M.; MARZBAN A.R.; FAKHARI J.

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2012 | Volume:4 | Issue:13 Page(s): 13-20

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Information Journal Paper

Title

STUDY OF CRUDE OIL BIOREMEDIATION BY BACTERIUM ISOLATED FROM SOILS CONTAMINATED WITH PETROLEUM HYDROCARBONS

Author(s)

EBRAHIMIPOUR GH. | KARKHANE M. | MARZBAN A.R. | FAKHARI J. | Issue Writer Certificate

Keywords

PLANOCOCCUSQ2

BIOREMEDIATIONQ2

MINERALIZATIONQ2

HYDROCARBON CONTAMINANTSQ2

CRUDE OILQ2

Abstract

Background and Objective: The pollutions of petroleum industries in environments are removed during relatively long time. Providing optimum conditions for oil degrading microorganisms would increase more efficiently removal rate of these contaminants. The aim of this study was to isolate the best oil degrading bacterium for applying on BIOREMEDIATION process.Materials and Methods: To find this objective, sampling was done from soils contaminated with CRUDE OIL and the best strain isolated and named Abt1. Then, oil degrading tests were performed under the different conditions.Results: Biochemical tests and 16s rDNA analysis showed that Abt1 belonged to PLANOCOCCUS with 100% similarity to a strain with Accession GU213131 in Genebank. This strain was able to grow at presence of 0-7% NaCl and tolerates pHs between5 to 8. The optimum conditions for petroleum biodegradation were pH 7.5, NaCl 0% and temperature around 30oC. Also minimum nitrogen and phosphate needed for degrading 1g CRUDE OIL were 0.292 and 0.036g/l respectively.Conclusion: This strain was able to degrade 1g CRUDE OIL during 12 days. Because the environments contaminated with oil hydrocarbons have the different salt contents, halotolerant microorganisms could increase treatment rate of contaminants. Therefore, this halotolerant strain could be used to remove pollutions from water and fields.

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APA: Copy

EBRAHIMIPOUR, GH., KARKHANE, M., MARZBAN, A.R., & FAKHARI, J.. (2012). STUDY OF CRUDE OIL BIOREMEDIATION BY BACTERIUM ISOLATED FROM SOILS CONTAMINATED WITH PETROLEUM HYDROCARBONS. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 4(13), 13-20. SID. https://sid.ir/paper/181465/en

Vancouver: Copy

EBRAHIMIPOUR GH., KARKHANE M., MARZBAN A.R., FAKHARI J.. STUDY OF CRUDE OIL BIOREMEDIATION BY BACTERIUM ISOLATED FROM SOILS CONTAMINATED WITH PETROLEUM HYDROCARBONS. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2012;4(13):13-20. Available from: https://sid.ir/paper/181465/en

IEEE: Copy

GH. EBRAHIMIPOUR, M. KARKHANE, A.R. MARZBAN, and J. FAKHARI, “STUDY OF CRUDE OIL BIOREMEDIATION BY BACTERIUM ISOLATED FROM SOILS CONTAMINATED WITH PETROLEUM HYDROCARBONS,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 4, no. 13, pp. 13–20, 2012, [Online]. Available: https://sid.ir/paper/181465/en

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