DESIGNING AND OPTIMIZATION OF INTERNAL CONTROL FOR HERPES SIMPLEX VIRUS PCR TEST

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOSLEMI E.; AKBARYAN A.; SHAHHOSSEINY M.H.; GHAHRI M.; GHOLAMI F.; MASSAHI S.; ELMI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2012 | Volume:4 | Issue:12 Page(s): 43-52

Download Full-Text

Persian Verion

View:

2,649

Download:

Cites:

Information Journal Paper

Title

DESIGNING AND OPTIMIZATION OF INTERNAL CONTROL FOR HERPES SIMPLEX VIRUS PCR TEST

Author(s)

Keywords

HERPES SIMPLEX VIRUSQ2

PCRQ2

INTERNAL CONTROLQ2

Abstract

Background and Objective: HERPES SIMPLEX VIRUS (HSV) is a member of the herpes virus family, Herpesviridae, which infect humans. There are different methods for detection of this virus like cultural ways which is time consuming, but molecular methods such as PCR are more appropriate for detection of HSV. Although nowadays molecular assays are using in laboratories but different results are achieved because of lack of standard methods which are counted as disadvantageous of molecular methods. For solving this issue in this study, designing of competitive INTERNAL CONTROL(IC) through PCR-cloning has been attempted.Materials and methods: primers for PCR test were optimized. Besides, the composite primers for IC-HSV were designed then the PCR was optimized. The IC-HSV which amplified, was ligated in pTZ57R plasmid then transformed in E.coli JM107 and was cloned. Specificity and sensitivity of test were determined.Results: PCR amplicon for HSV and IC-HSV with special primers were 454bp and 662bp respectively, so there was a significant different between their size. The appropriate concentration of IC used in each reaction was 50 fg and the sensivity of HSV DNA with IC was between 1 million to 1000 virus particles.Conclusion: Despite of high speed and accuracy of PCR, false positive and negative results which are caused by PCR inhibitors, are the most important problems of this technique that can reduce its efficiency. Using another DNA as an INTERNAL CONTROL can detect these inhibitors. Indeed, amplification of this DNA shows correct amplification and detection steps.

Cites

No record.

References

No record.

Cite

APA: Copy

MOSLEMI, E., AKBARYAN, A., SHAHHOSSEINY, M.H., GHAHRI, M., GHOLAMI, F., MASSAHI, S., & ELMI, M.. (2012). DESIGNING AND OPTIMIZATION OF INTERNAL CONTROL FOR HERPES SIMPLEX VIRUS PCR TEST. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 4(12), 43-52. SID. https://sid.ir/paper/181474/en

Vancouver: Copy

MOSLEMI E., AKBARYAN A., SHAHHOSSEINY M.H., GHAHRI M., GHOLAMI F., MASSAHI S., ELMI M.. DESIGNING AND OPTIMIZATION OF INTERNAL CONTROL FOR HERPES SIMPLEX VIRUS PCR TEST. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2012;4(12):43-52. Available from: https://sid.ir/paper/181474/en

IEEE: Copy

E. MOSLEMI, A. AKBARYAN, M.H. SHAHHOSSEINY, M. GHAHRI, F. GHOLAMI, S. MASSAHI, and M. ELMI, “DESIGNING AND OPTIMIZATION OF INTERNAL CONTROL FOR HERPES SIMPLEX VIRUS PCR TEST,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 4, no. 12, pp. 43–52, 2012, [Online]. Available: https://sid.ir/paper/181474/en

Related Journal Papers