MONOSPECIFIC POLYCLONAL ANTISERUM PRODUCTION AGAINST BACTERIAL HBSU

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GHADAM P.; GHODSI S.; BANDEHPOUR M.; BANIJAMALI M.

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2011 | Volume:2 | Issue:7 Page(s): 17-22

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Title

MONOSPECIFIC POLYCLONAL ANTISERUM PRODUCTION AGAINST BACTERIAL HBSU

Author(s)

GHADAM P. | GHODSI S. | BANDEHPOUR M. | BANIJAMALI M. | Issue Writer Certificate

Keywords

LIKE PROTEIN HBSUQ3

BACILLUS SUBTILISQ2

MONOSPECIFIC POLYCLONAL ANTISERUMQ3

Abstract

Background and Objective: HBsu protein in BACILLUS SUBTILIS is a member of histone like protein family HU. The protein has an important role in structure and function of bacterial nucleoid. In sights of its importance and similarity between the members of HU family, in this study the purified expressed HBsu, as an immunogen, was injected in rabbit until the produced polyclonal antiserum was used in the next research about identification of the other HU family members.Materials and Methods: The recently expressed HBsu protein from hbs gene of BACILLUS SUBTILIS ATCC6633 was injected in two male rabbits according to a twenty-day schedule and immunoblotting was done for antiserum production checking.Results: After twenty days, the antiserum against HBsu was produced and the presence of the antiserum was confirmed by immunoblotting. Also, extracted histone like protein from Halobacillus karajenesis was used for checking of its ability in identification of HBsu like protein that the answer was positive.Conclusion: The used method was rapid and with high ability for antiserum production against HBsu protein. The similarity between Halobacillus karajenesis HU and BACILLUS SUBTILIS HBsu is enough for the identification of this HU by antiserum against HBsu.

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APA: Copy

GHADAM, P., GHODSI, S., BANDEHPOUR, M., & BANIJAMALI, M.. (2011). MONOSPECIFIC POLYCLONAL ANTISERUM PRODUCTION AGAINST BACTERIAL HBSU. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 2(7), 17-22. SID. https://sid.ir/paper/181490/en

Vancouver: Copy

GHADAM P., GHODSI S., BANDEHPOUR M., BANIJAMALI M.. MONOSPECIFIC POLYCLONAL ANTISERUM PRODUCTION AGAINST BACTERIAL HBSU. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2011;2(7):17-22. Available from: https://sid.ir/paper/181490/en

IEEE: Copy

P. GHADAM, S. GHODSI, M. BANDEHPOUR, and M. BANIJAMALI, “MONOSPECIFIC POLYCLONAL ANTISERUM PRODUCTION AGAINST BACTERIAL HBSU,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 2, no. 7, pp. 17–22, 2011, [Online]. Available: https://sid.ir/paper/181490/en

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