OPTIMIZING OF DIPHTHERIA TOXIN PRODUCTION BY USING OF TAGUCHI METHOD

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KAZEMNEJAD ANOUSHIRAVAN; MOVAHEDI A.A.R.; SALEHI MASOUD

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Journal: Year:2002 | Volume:14 | Issue:4 (53 IN ANIMAL & FISHRIES SCIENCES) Page(s): 54-57

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Title

OPTIMIZING OF DIPHTHERIA TOXIN PRODUCTION BY USING OF TAGUCHI METHOD

Author(s)

KAZEMNEJAD ANOUSHIRAVAN | MOVAHEDI A.A.R. | SALEHI MASOUD | Issue Writer Certificate

Keywords

DIPHTHERIA TOXIN PRODUCTIONQ4

STRAINER MEDIUMQ4

OPTIMAL DESIGNQ3

TAGUCHI METHODQ4

Abstract

For large scale production of diphtheria toxin, Coryneabactrium diphtheria must be cultured in a nutrient medium, and optimum conditions. In this condition coryneabactrium produces maximum amount of toxin, which is used for, diphtheria vaccine production. For obtaining maximum yield, needed to find the optimum conditions and use a suitable statistical design 4 to determine the effects of factors and errors. For this reason in different statistical experiment designs we used orthogonal TAGUCHI METHOD. For this expects the orthogonal array L'16 was used for the following five factors: Concentrations of 1. N. Z Amine (gr/lit), 2. Iron (mg/lit), 3 Calcium, (mg/lit), 4. phosphate solution (ml/lit), 5. Growth factors solution (ml/lit) that all of them had four levels. Optimum condition and optimum response determined after analysis of experiment results. Final results showed in the optimum condition, toxin production level increased from 120lf/ml (over 60%) and comparable to other vaccine manufacturing of developed countries.

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APA: Copy

KAZEMNEJAD, ANOUSHIRAVAN, MOVAHEDI, A.A.R., & SALEHI, MASOUD. (2002). OPTIMIZING OF DIPHTHERIA TOXIN PRODUCTION BY USING OF TAGUCHI METHOD. PAJOUHESH-VA-SAZANDEGI, 14(4 (53 IN ANIMAL & FISHRIES SCIENCES)), 54-57. SID. https://sid.ir/paper/18744/en

Vancouver: Copy

KAZEMNEJAD ANOUSHIRAVAN, MOVAHEDI A.A.R., SALEHI MASOUD. OPTIMIZING OF DIPHTHERIA TOXIN PRODUCTION BY USING OF TAGUCHI METHOD. PAJOUHESH-VA-SAZANDEGI[Internet]. 2002;14(4 (53 IN ANIMAL & FISHRIES SCIENCES)):54-57. Available from: https://sid.ir/paper/18744/en

IEEE: Copy

ANOUSHIRAVAN KAZEMNEJAD, A.A.R. MOVAHEDI, and MASOUD SALEHI, “OPTIMIZING OF DIPHTHERIA TOXIN PRODUCTION BY USING OF TAGUCHI METHOD,” PAJOUHESH-VA-SAZANDEGI, vol. 14, no. 4 (53 IN ANIMAL & FISHRIES SCIENCES), pp. 54–57, 2002, [Online]. Available: https://sid.ir/paper/18744/en

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