Optimization of diphtheria toxoid precipitation by using saturated ammonium sulphate in order to improve diphtheria vaccine

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Akrami Meisam; NOOFELI MOJTABA; Tabatabaei Mitra Sadat

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2018 | Volume:8 | Issue:31 Page(s): 95-102

Download Full-Text

Persian Verion

View:

1,379

Download:

Cites:

Information Journal Paper

Title

Optimization of diphtheria toxoid precipitation by using saturated ammonium sulphate in order to improve diphtheria vaccine

Author(s)

Akrami Meisam | NOOFELI MOJTABA | Tabatabaei Mitra Sadat | Issue Writer Certificate

Keywords

Not Registered.

Abstract

Aim and Background: Diphtheria toxin is an extracellular protein of Corynebacterium diphtheria that inhibits protein synthesis and kills susceptible cells. Ammonium sulfate is an inorganic salt that is used to purify and precipitate proteins, which, by increasing salt content, decreases the solubility of proteins, and proteins interconnect and precipitate through their hydrophobic parts. Diphtheria vaccine is prepared by purifying and treating toxoid diphtheria bacteria, which is one of the methods of treating, using ammonium sulfate as a dry salt or preparing an over-saturated solution. Material and Methods: Diphtheria bacteria grown in optimal conditions and increased nutrient and toxin production by bacteria and inactivating the toxin into toxoid, in the manufacture of the vaccine is use. All media must be at least 24 hours at a temperature of 37-35° C will be incubated. In order to bacterial toxoid deposition of supersaturated solution 4. 32 M ammonium sulfate with different percentages were used. The results of this procedure were investigated using SDS PAGE and Lf TEST ( Lateral Flow Test). Results: Results revealed the use of 4. 32 M supersaturated ammonium sulfate instead of the use of dry salt of ammonium sulfate, used in the traditional method (WHO TRS, No. 980), used previously in the vaccine industry, Eliminating the long-term dialysis phase On the other hand, the final product (toxoid) has been tediously reduced It also removes unwanted impurities from the finished product And has made the net product even more scalable. Conclusion: Since the purpose of vaccine production is industry to achieve maximum toxoid of the bacteria, so use the procedure in this research project (optimizing of DT toxin precipitation by 4. 32 M super saturation of ammonium sulphate), in addition to reducing the loss of the final product and remove the prolonged dialysis

Cites

No record.

References

No record.

Cite

APA: Copy

Akrami, Meisam, NOOFELI, MOJTABA, & Tabatabaei, Mitra Sadat. (2018). Optimization of diphtheria toxoid precipitation by using saturated ammonium sulphate in order to improve diphtheria vaccine. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 8(31 ), 95-102. SID. https://sid.ir/paper/204706/en

Vancouver: Copy

Akrami Meisam, NOOFELI MOJTABA, Tabatabaei Mitra Sadat. Optimization of diphtheria toxoid precipitation by using saturated ammonium sulphate in order to improve diphtheria vaccine. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2018;8(31 ):95-102. Available from: https://sid.ir/paper/204706/en

IEEE: Copy

Meisam Akrami, MOJTABA NOOFELI, and Mitra Sadat Tabatabaei, “Optimization of diphtheria toxoid precipitation by using saturated ammonium sulphate in order to improve diphtheria vaccine,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 8, no. 31 , pp. 95–102, 2018, [Online]. Available: https://sid.ir/paper/204706/en

Related Journal Papers