CLONING AND EXPRESSION OF RECOMBINANT IMMUNOTOXIN USING DIPHTHERIA TOXIN AND GRANULOCYTE COLONY STIMULATING FACTOR (G-CSF)

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DONYAPOOR FATEMEH; ZEINODDINI MEHDI; SAEEDINIA ALI REZA

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Journal: ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ) Year:2016 | Volume:19 | Issue:5 (110) Page(s): 42-50

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Title

CLONING AND EXPRESSION OF RECOMBINANT IMMUNOTOXIN USING DIPHTHERIA TOXIN AND GRANULOCYTE COLONY STIMULATING FACTOR (G-CSF)

Author(s)

DONYAPOOR FATEMEH | ZEINODDINI MEHDI | SAEEDINIA ALI REZA | Issue Writer Certificate

Keywords

IMMUNOTOXINQ1

DIPHTHERIA TOXINQ1

GRANULOCYT COLONY STIMULATING FACTORQ1

PROTEIN EXPRESSIONQ1

Abstract

Background: IMMUNOTOXIN (IT) is a directed toxin containing two distinct sections (immune and toxin parts) covalently bond using specific chemical or peptide linkers. The aim of this study is to produce a recombinant and hybrid protein containing DIPHTHERIA TOXIN (DT) and granulocyte colony stimulating factor (G-CSF).Materials and Methods: According to the structure of the first commercial recombinant IMMUNOTOXIN (Ontak, hybrid protein containing DT fused interlukine2), gene encoding of DT and GCSF was amplified using specific primers and plasmid template of pET-IDZ3 (pET21 harboring the gene encoding ontak IMMUNOTOXINe) and pET-GCSF (pET23 harboring G-CSF), respectively. The DT-GCSF fusion protein produced using soeing PCR and specific primers. Finally, pET-DT-GCSF construction prepared using subcloning of DT-GCSF into pET21a (+) and confirmed by sequencing, SDS-PAGE and western blot technique.Results: Gene encoding of DT-GCSF inserted into NdeI/EcoRI site of pET21 and the construction of strain producing DT-GCSF recombinant IMMUNOTOXIN was confirmed using customary methods.Conclusion: The cytokine fusion protein, DT-GCSF, could be used for the inhibition of GCSF receptor bearing cancer cells.

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APA: Copy

DONYAPOOR, FATEMEH, ZEINODDINI, MEHDI, & SAEEDINIA, ALI REZA. (2016). CLONING AND EXPRESSION OF RECOMBINANT IMMUNOTOXIN USING DIPHTHERIA TOXIN AND GRANULOCYTE COLONY STIMULATING FACTOR (G-CSF). ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 19(5 (110)), 42-50. SID. https://sid.ir/paper/68931/en

Vancouver: Copy

DONYAPOOR FATEMEH, ZEINODDINI MEHDI, SAEEDINIA ALI REZA. CLONING AND EXPRESSION OF RECOMBINANT IMMUNOTOXIN USING DIPHTHERIA TOXIN AND GRANULOCYTE COLONY STIMULATING FACTOR (G-CSF). ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2016;19(5 (110)):42-50. Available from: https://sid.ir/paper/68931/en

IEEE: Copy

FATEMEH DONYAPOOR, MEHDI ZEINODDINI, and ALI REZA SAEEDINIA, “CLONING AND EXPRESSION OF RECOMBINANT IMMUNOTOXIN USING DIPHTHERIA TOXIN AND GRANULOCYTE COLONY STIMULATING FACTOR (G-CSF),” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 19, no. 5 (110), pp. 42–50, 2016, [Online]. Available: https://sid.ir/paper/68931/en

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