EVALUATION OF VIABILITY AND APOPTOSIS IN HUMAN SPERM TREATED WITH SODIUM ARSENITE

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MOMENI H.R.; SOLEIMANI MEHRANJANI M.; ABNOSI M.H.; BAKHTIARI A.; ESKANDARI N.; RAFEIE M.

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Journal: JOURNAL OF CELL & TISSUE Year:2014 | Volume:4 | Issue:4 Page(s): 361-370

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Title

EVALUATION OF VIABILITY AND APOPTOSIS IN HUMAN SPERM TREATED WITH SODIUM ARSENITE

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Keywords

HUMAN SPERMQ2

APOPTOSISQ2

DNA INTEGRITYQ2

SODIUM ARSENITEQ2

SPERM VIABILITYQ2

Abstract

Aim: The aim of this research was to evaluate viability as well as morphological and biochemical featears of APOPTOSIS in HUMAN SPERM treated with sodium arseinte.Material and methods: HUMAN SPERM samples were exposed with different concentration of SODIUM ARSENITE (0, 0.1, 20 and 100 mM) for 0, 60, 120 and 180 minutes. SPERM VIABILITY was done using MTT assay and sperm DNA INTEGRITY was investigated by acridine orange staining. Morphological feature of APOPTOSIS in sperm nucleus was studied using Hoechst and Diff-Quick staining, whereas biochemical feature of APOPTOSIS was studied by TUNEL method. Statistical analysis was carried out by one-way ANOVA and repeated measure.Results: MTT assay showed a significant interaction between SODIUM ARSENITE concentration and time on the decrease SPERM VIABILITY. In addition, after 180 minutes, the viability of sperm treated with SODIUM ARSENITE was significantly decreased compared to control. However, this pollutant had no effect on DNA INTEGRITY as well as morphological and biochemical features of APOPTOSIS in the sperm nucleus.Conclusion: SODIUM ARSENITE caused a significant decrease in HUMAN SPERM viability and this effect was not due to the damage of sperm nucleus and DNA.

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APA: Copy

MOMENI, H.R., SOLEIMANI MEHRANJANI, M., ABNOSI, M.H., BAKHTIARI, A., ESKANDARI, N., & RAFEIE, M.. (2014). EVALUATION OF VIABILITY AND APOPTOSIS IN HUMAN SPERM TREATED WITH SODIUM ARSENITE. JOURNAL OF CELL & TISSUE, 4(4), 361-370. SID. https://sid.ir/paper/188924/en

Vancouver: Copy

MOMENI H.R., SOLEIMANI MEHRANJANI M., ABNOSI M.H., BAKHTIARI A., ESKANDARI N., RAFEIE M.. EVALUATION OF VIABILITY AND APOPTOSIS IN HUMAN SPERM TREATED WITH SODIUM ARSENITE. JOURNAL OF CELL & TISSUE[Internet]. 2014;4(4):361-370. Available from: https://sid.ir/paper/188924/en

IEEE: Copy

H.R. MOMENI, M. SOLEIMANI MEHRANJANI, M.H. ABNOSI, A. BAKHTIARI, N. ESKANDARI, and M. RAFEIE, “EVALUATION OF VIABILITY AND APOPTOSIS IN HUMAN SPERM TREATED WITH SODIUM ARSENITE,” JOURNAL OF CELL & TISSUE, vol. 4, no. 4, pp. 361–370, 2014, [Online]. Available: https://sid.ir/paper/188924/en

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