THE EFFECT OF MEDIUM, CARBON SOURCE, LIGHT SPECTRUM AND STYLE TREATMENT OF AUXIN ON SHOOT AND ROOT REGENERATION OF GISELA 6 ROOT STOCK

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ZAREI M.; GAROOSI GH.; NEZAMI E.; HOSSEINI R.; AHMADI J.

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Journal: JOURNAL OF CELL & TISSUE Year:2013 | Volume:4 | Issue:2 Page(s): 169-185

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Title

THE EFFECT OF MEDIUM, CARBON SOURCE, LIGHT SPECTRUM AND STYLE TREATMENT OF AUXIN ON SHOOT AND ROOT REGENERATION OF GISELA 6 ROOT STOCK

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Keywords

CARBON SOURCEQ2

GISELA 6Q2

LIGHT SPECTRUMQ1

ROOTINGQ2

SHOOT REGENERATIONQ2

Abstract

Aim: In order to localize and optimize of micro propagation of GISELA 6, a valuable rootstock, the effects of medium, CARBON SOURCEs, light and method of auxin application on shooting and ROOTING were investigated.Material and methods: The effect of six media, five CARBON SOURCEs and three light spectra plus different concentrations of BA and IBA on growth characters including SHOOT REGENERATION and their length, were studied using lateral buds as explants.. Percentage of ROOTING, number and length of roots were surveyed in two methods: 1) pulsing method using IBA and NAA at 1.0 g.L-1 for different times; and 2) shoot planting on solid medium containing 6 concentrations of IBA and NAA independently.Results: MS was the most suitable medium and the highest and longest shootlet numbers were produced on medium containing 30 gr. L-1 of market sugar and sucrose respectively. White and red light are more effective on SHOOT REGENERATION and shoot length respectively. The highest root induction was occurred in pulsing method in presence of 1 g. L-1 of IBA.Conclusion: Optimized shoot propagation was observed on MS medium containing market sugar and BAP under white light, and optimized root induction was occurred in pulsing method in presence of IBA.

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APA: Copy

ZAREI, M., GAROOSI, GH., NEZAMI, E., HOSSEINI, R., & AHMADI, J.. (2013). THE EFFECT OF MEDIUM, CARBON SOURCE, LIGHT SPECTRUM AND STYLE TREATMENT OF AUXIN ON SHOOT AND ROOT REGENERATION OF GISELA 6 ROOT STOCK. JOURNAL OF CELL & TISSUE, 4(2), 169-185. SID. https://sid.ir/paper/188965/en

Vancouver: Copy

ZAREI M., GAROOSI GH., NEZAMI E., HOSSEINI R., AHMADI J.. THE EFFECT OF MEDIUM, CARBON SOURCE, LIGHT SPECTRUM AND STYLE TREATMENT OF AUXIN ON SHOOT AND ROOT REGENERATION OF GISELA 6 ROOT STOCK. JOURNAL OF CELL & TISSUE[Internet]. 2013;4(2):169-185. Available from: https://sid.ir/paper/188965/en

IEEE: Copy

M. ZAREI, GH. GAROOSI, E. NEZAMI, R. HOSSEINI, and J. AHMADI, “THE EFFECT OF MEDIUM, CARBON SOURCE, LIGHT SPECTRUM AND STYLE TREATMENT OF AUXIN ON SHOOT AND ROOT REGENERATION OF GISELA 6 ROOT STOCK,” JOURNAL OF CELL & TISSUE, vol. 4, no. 2, pp. 169–185, 2013, [Online]. Available: https://sid.ir/paper/188965/en

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