SEQUENCING OF LEAFY HOMOLOGOUS GENE IN SOLANUM VILLOSUM MILL.

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ASADI KHANUKI M.; REZANEJAD F.; SHARIFI SIRCHI G.R.; SASAN H.A.

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Journal: JOURNAL OF CELL & TISSUE Year:2015 | Volume:6 | Issue:1 Page(s): 51-58

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Title

SEQUENCING OF LEAFY HOMOLOGOUS GENE IN SOLANUM VILLOSUM MILL.

Author(s)

ASADI KHANUKI M. | REZANEJAD F. | SHARIFI SIRCHI G.R. | SASAN H.A. | Issue Writer Certificate

Keywords

FLOWERINGQ2

LEAFYQ2

RT-PCRQ2

SOLANUM VILLOSUM MILL.Q2

Abstract

Aim: The purpose of this study was to identify LEAFY homologous genes in a type of nightshade (Solanum villosum Mill.).Material and Methods: Total RNA was isolated from shoot apical meristem tissue of S. villosum and was used for First-strand cDNA synthesis. The specific primers were designed based on nucleotide sequence alignment of LEAFY homologous genes from other plants and were used in RT-PCR. The RT-PCR products were purified and sequenced.Results: The results indicated amplification of 540 nucleotides fragment, and named SvLFY. Comparison of SvLFY deduced protein sequences with LEAFY homologous proteins from other species showed high similarity between this fragment and in other C-terminal regions.Conclusion: These results suggest that SvLFY may play a similar role as LEAFY in flower development and could act as a flower meristem identity gene in SOLANUM VILLOSUM MILL.

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APA: Copy

ASADI KHANUKI, M., REZANEJAD, F., SHARIFI SIRCHI, G.R., & SASAN, H.A.. (2015). SEQUENCING OF LEAFY HOMOLOGOUS GENE IN SOLANUM VILLOSUM MILL.. JOURNAL OF CELL & TISSUE, 6(1), 51-58. SID. https://sid.ir/paper/189006/en

Vancouver: Copy

ASADI KHANUKI M., REZANEJAD F., SHARIFI SIRCHI G.R., SASAN H.A.. SEQUENCING OF LEAFY HOMOLOGOUS GENE IN SOLANUM VILLOSUM MILL.. JOURNAL OF CELL & TISSUE[Internet]. 2015;6(1):51-58. Available from: https://sid.ir/paper/189006/en

IEEE: Copy

M. ASADI KHANUKI, F. REZANEJAD, G.R. SHARIFI SIRCHI, and H.A. SASAN, “SEQUENCING OF LEAFY HOMOLOGOUS GENE IN SOLANUM VILLOSUM MILL.,” JOURNAL OF CELL & TISSUE, vol. 6, no. 1, pp. 51–58, 2015, [Online]. Available: https://sid.ir/paper/189006/en

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