CYTOTOXICITY OF CERIUM OXIDE NANOPARTICLES (CEO2) ON COLON CANCER CELL LINE (HT29) AND EVALUATION OF CASPASE 3 AND 9 APOPTOSIS GENE EXPRESSION USING REAL TIME PCR AND FLOW-CYTOMETRY METHODS

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NASIRI R.; ZARE KARIZI SH.; HAYATI ROODBARI N.; FARHADYAR N.

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Journal: JOURNAL OF CELL & TISSUE Year:2017 | Volume:8 | Issue:3 Page(s): 294-303

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Title

CYTOTOXICITY OF CERIUM OXIDE NANOPARTICLES (CEO2) ON COLON CANCER CELL LINE (HT29) AND EVALUATION OF CASPASE 3 AND 9 APOPTOSIS GENE EXPRESSION USING REAL TIME PCR AND FLOW-CYTOMETRY METHODS

Author(s)

NASIRI R. | ZARE KARIZI SH. | HAYATI ROODBARI N. | FARHADYAR N. | Issue Writer Certificate

Keywords

CASPASE 3 AND 9Q1

CERIUM OXIDE NANOPARTICLESQ1

HT29 CELL LINEQ1

Abstract

Aim: In the current study, effect of cerium oxide nano-particles cytotoxicity was investigated on the colon cancer cell line HT29 and evaluation of CASPASE 3 AND 9 apoptosis genes expression.Material and Methods: In this experimental study, the HT29 CELL LINE was treated with different concentrations of cerium oxide nano-particles, including 0.78, 1.56, 3.12, 6.25, 12.5,25 50 and 100 mg/ml in 24, 48 and 72 hours and the cell viability was determined using MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide ) test. Then, the gene expression level of CASPASE 3 AND 9 genes was evaluated by using Real Time PCR in treated HT29 CELL LINE with IC50 value during 24 h. Finally, the apoptosis induction was assessed by flow-cytometry.Results: The MTT results show that cerium oxide nano-particles had maximum cytotoxicity on HT29 CELL LINE in 25, 50 and 100 mg/ml concentrations in 72 h. Moreover, the Real Time PCR results indicated that the relative expression level of CASPASE 3 AND 9 was up-regulated significantly (2.36±0.76 and 3.4±.95, respectively) in HT29 CELL LINE treated with nano-particle. The flow-cytometry results revealed the 16% apoptosis in HT29 CELL LINE.Conclusion: Findings showed that the cytotoxicity of cerium oxide was based on time and applied dose. Thus, it appears that this nano-particle could be used in pharmaceutical applications with further studies about selective targeting of cerium oxide nano-particles.

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APA: Copy

NASIRI, R., ZARE KARIZI, SH., HAYATI ROODBARI, N., & FARHADYAR, N.. (2017). CYTOTOXICITY OF CERIUM OXIDE NANOPARTICLES (CEO2) ON COLON CANCER CELL LINE (HT29) AND EVALUATION OF CASPASE 3 AND 9 APOPTOSIS GENE EXPRESSION USING REAL TIME PCR AND FLOW-CYTOMETRY METHODS. JOURNAL OF CELL & TISSUE, 8(3 ), 294-303. SID. https://sid.ir/paper/189085/en

Vancouver: Copy

NASIRI R., ZARE KARIZI SH., HAYATI ROODBARI N., FARHADYAR N.. CYTOTOXICITY OF CERIUM OXIDE NANOPARTICLES (CEO2) ON COLON CANCER CELL LINE (HT29) AND EVALUATION OF CASPASE 3 AND 9 APOPTOSIS GENE EXPRESSION USING REAL TIME PCR AND FLOW-CYTOMETRY METHODS. JOURNAL OF CELL & TISSUE[Internet]. 2017;8(3 ):294-303. Available from: https://sid.ir/paper/189085/en

IEEE: Copy

R. NASIRI, SH. ZARE KARIZI, N. HAYATI ROODBARI, and N. FARHADYAR, “CYTOTOXICITY OF CERIUM OXIDE NANOPARTICLES (CEO2) ON COLON CANCER CELL LINE (HT29) AND EVALUATION OF CASPASE 3 AND 9 APOPTOSIS GENE EXPRESSION USING REAL TIME PCR AND FLOW-CYTOMETRY METHODS,” JOURNAL OF CELL & TISSUE, vol. 8, no. 3 , pp. 294–303, 2017, [Online]. Available: https://sid.ir/paper/189085/en

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