STUDY THE EFFECT OF PLL POLYMER ON GENE DELIVERY EFFICIENCY BY BIODEGRADABLE PLA-PEG DI-BLOCK COPOLYMERS

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MOSAVI SOOHA S.A.; YAGHOUBI H.

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Journal: JOURNAL OF CELL & TISSUE Year:2017 | Volume:8 | Issue:3 Page(s): 271-284

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Title

STUDY THE EFFECT OF PLL POLYMER ON GENE DELIVERY EFFICIENCY BY BIODEGRADABLE PLA-PEG DI-BLOCK COPOLYMERS

Author(s)

MOSAVI SOOHA S.A. | YAGHOUBI H. | Issue Writer Certificate

Keywords

PLA-PEGQ1

PLLQ1

BIOCOMPATIBILITYQ1

DNA RELEASEQ1

GENE DELIVERYQ1

Abstract

Aim: The present study aims to investigate the effect of simultaneous encapsulation of DNA with different ratio of PLL via PLA-PEG copolymer, on GENE DELIVERY efficiency into mammalian cells. Some characteristics such as BIOCOMPATIBILITY, DNA protecting against restriction enzymes, DNA RELEASE rate, size and zeta potential were also investigated.Material and Methods: PLA-PEG/PLL/DNA nanoparticles with different ratio of PLL were prepared by double emulsion-solvent evaporation technique. Then, the release percentage of DNA and the zeta potential of particles in Phosphate Saline buffer (PH=7) were measured.Results: Increasing the PLL percentage in the PLA-PEG/PLL/DNA nanoparticles resulted in enhancement of particles size and zeta potential. Gel electrophoresis analysis of DNA extracted from PLA-PEG/DNA and PLA-PEG/PLL/DNA nanoparticles after treatment with DNase I enzyme indicates the ability of PLA-PEG and PLA-PEG/PLL copolymers in DNA protection. The flow cytometry and fluorescent microscopy study confirmed that gene transfer efficiency was improved by increasing the ratio of PLL in PLA-PEG/PLL/DNA nanoparticles. Moreover we found that gene transfer efficiency was one and half fold higher then PLL/DNA complex in the serum medium.Conclusion: The results showed that PLA-PEG nanoparticles in the serum medium have higher potential GENE DELIVERY to the MCF-7 cells in comparison with PLA-PEG/PLL/DNA nanoparticles.

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APA: Copy

MOSAVI SOOHA, S.A., & YAGHOUBI, H.. (2017). STUDY THE EFFECT OF PLL POLYMER ON GENE DELIVERY EFFICIENCY BY BIODEGRADABLE PLA-PEG DI-BLOCK COPOLYMERS. JOURNAL OF CELL & TISSUE, 8(3 ), 271-284. SID. https://sid.ir/paper/189091/en

Vancouver: Copy

MOSAVI SOOHA S.A., YAGHOUBI H.. STUDY THE EFFECT OF PLL POLYMER ON GENE DELIVERY EFFICIENCY BY BIODEGRADABLE PLA-PEG DI-BLOCK COPOLYMERS. JOURNAL OF CELL & TISSUE[Internet]. 2017;8(3 ):271-284. Available from: https://sid.ir/paper/189091/en

IEEE: Copy

S.A. MOSAVI SOOHA, and H. YAGHOUBI, “STUDY THE EFFECT OF PLL POLYMER ON GENE DELIVERY EFFICIENCY BY BIODEGRADABLE PLA-PEG DI-BLOCK COPOLYMERS,” JOURNAL OF CELL & TISSUE, vol. 8, no. 3 , pp. 271–284, 2017, [Online]. Available: https://sid.ir/paper/189091/en

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