Assessment of Cytotoxicity of Bacillus anthracis Recombinant Protective Antigen in Free and Encapsulated Forms by Double-block PLA-PEG and PCL-PEG Copolymers on Vero Cell

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ETEMAD AUBI SEYED MASIH; HONARI HOSEIN; Hajinourmohamadi Ashkan; BAGHERI HAMED; NOOFELI MOJTABA

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Journal: Jundishapur Scientific Medical Journal Year:2018 | Volume:17 | Issue:4 Page(s): 377-386

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Title

Assessment of Cytotoxicity of Bacillus anthracis Recombinant Protective Antigen in Free and Encapsulated Forms by Double-block PLA-PEG and PCL-PEG Copolymers on Vero Cell

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Keywords

Bacillus anthracisQ2

Protective antigenQ1

Cell cytotoxicityQ1

PLA-PEGQ2

PCL-PEGQ1

Abstract

Background and Objectives: Bacillus anthracis is the cause of the fatal anthrax. Use of vaccine is one of the effective ways to combat anthrax. Vaccines should be biocompatible and non-toxic. The purpose of this study was to investigate the cytotoxicity effect of free and encapsulated forms of Bacillus anthraise recombinant Protective antigen by double-block PLA-PEG and PCL-PEG copolymers on the epithelial cell line of the African green monkey (Vero). Subjects and Methods: In this experimental study, the Bacillus anthraise recombinant Protective antigen (PA63) was expressed in E. coli and purified by nickel column affinity chromatography. Subsequently PA63 was encapsulated by PLA-PEG and PCL-PEG double-block copolymers using water-in-oil-in-water solvent evaporation method. Finally, PA63 and nanoparticles cytotoxicity test were performed using the standard MTT test on the Vero cell line. Results: Results of the MTT test revealed the non-toxicity of the candidate vaccine. Also, the mortality rate of the cells tested against the encapsulated antigen in the nanoparticles was lower than the free antigen. Conclusion: The nano-vaccine formulation can be used to reduce the toxicity of a vaccine produced and eventually acquire an effective and biocompatible recombinant vaccine.

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APA: Copy

ETEMAD AUBI, SEYED MASIH, HONARI, HOSEIN, Hajinourmohamadi, Ashkan, BAGHERI, HAMED, & NOOFELI, MOJTABA. (2018). Assessment of Cytotoxicity of Bacillus anthracis Recombinant Protective Antigen in Free and Encapsulated Forms by Double-block PLA-PEG and PCL-PEG Copolymers on Vero Cell. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, 17(4 ), 377-386. SID. https://sid.ir/paper/408374/en

Vancouver: Copy

ETEMAD AUBI SEYED MASIH, HONARI HOSEIN, Hajinourmohamadi Ashkan, BAGHERI HAMED, NOOFELI MOJTABA. Assessment of Cytotoxicity of Bacillus anthracis Recombinant Protective Antigen in Free and Encapsulated Forms by Double-block PLA-PEG and PCL-PEG Copolymers on Vero Cell. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL[Internet]. 2018;17(4 ):377-386. Available from: https://sid.ir/paper/408374/en

IEEE: Copy

SEYED MASIH ETEMAD AUBI, HOSEIN HONARI, Ashkan Hajinourmohamadi, HAMED BAGHERI, and MOJTABA NOOFELI, “Assessment of Cytotoxicity of Bacillus anthracis Recombinant Protective Antigen in Free and Encapsulated Forms by Double-block PLA-PEG and PCL-PEG Copolymers on Vero Cell,” JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, vol. 17, no. 4 , pp. 377–386, 2018, [Online]. Available: https://sid.ir/paper/408374/en

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