INVESTIGATING OF PROTECTIVE EFFECTS OF NURR1 AND GDNF ON DOPAMINERGIC NEURAL CELL LINE SH-SY5Y AGAINST NEURO-INFLAMMATION AND TOXICITY OF 6-OHDA

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RASOOLNEZHAD M.; GARDANEH M.; SABOUNI F.

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Journal: JOURNAL OF CELL & TISSUE Year:2017 | Volume:8 | Issue:1 Page(s): 87-108

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Title

INVESTIGATING OF PROTECTIVE EFFECTS OF NURR1 AND GDNF ON DOPAMINERGIC NEURAL CELL LINE SH-SY5Y AGAINST NEURO-INFLAMMATION AND TOXICITY OF 6-OHDA

Author(s)

RASOOLNEZHAD M. | GARDANEH M. | SABOUNI F. | Issue Writer Certificate

Keywords

GDNFQ1

MICROGLIAQ1

NEUROINFLAMMATIONQ2

NURR1Q1

OXIDATIVE STRESSQ1

Abstract

Aim: In this study NURR1 and GDNF, due to their anti-inflammatory and regenerative effects and NURR1-mediated regulation of GDNF receptor (Ret) expression, were selected to protect dopaminergic SH-SY5Y cell line against NEUROINFLAMMATION and toxicity caused by 6-OHDA.Material and Methods: Recombinant lentiviral vectors carrying NURR1 and GDNF genes were prepared and transdused to SH-SY5Y and astrocytoma (1321N1) cell lines respectively. Also HEK-293T cells were transfected with plasmid carrying GDNF to overexpress this factor, condition media of transduced astrocytoma and transfected HEK-293T cells were collected and stored. Next, overexpression of mentioned factors was demonstrated by RT-PCR. On the other hand, MICROGLIAl cells were isolated from neonatal rat brains and induced with LPS to produce neuroinflammatory factors, Inducible expression of them was demonstrated by Griess test and RT-PCR. Condition media of MICROGLIA was collected and saved. Finally, SH-SY5Y cells overexpressing NURR1 were treated with condition media of transduced astrocytoma / transfected HEK-293T and then with condition media of LPS-induced MICROGLIA or 6-OHDA toxin.Results: data from MTT assay showed, SH-SY5Y cells overexpressing NURR1 or pretreated with GDNF are more resistant to toxicity caused by NEUROINFLAMMATION and 6-OHDA. Also NURR1 and GDNF have cooperative effects and give more protection to dopaminergic cells.Conclusion: NURR1 and GDNF each have protective effects on dopaminergic neural cells against inflammatory factors or 6-OHDA. Also they synergize with each other leading to more protection for dopaminergic neural cells.

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APA: Copy

RASOOLNEZHAD, M., GARDANEH, M., & SABOUNI, F.. (2017). INVESTIGATING OF PROTECTIVE EFFECTS OF NURR1 AND GDNF ON DOPAMINERGIC NEURAL CELL LINE SH-SY5Y AGAINST NEURO-INFLAMMATION AND TOXICITY OF 6-OHDA. JOURNAL OF CELL & TISSUE, 8(1 ), 87-108. SID. https://sid.ir/paper/189131/en

Vancouver: Copy

RASOOLNEZHAD M., GARDANEH M., SABOUNI F.. INVESTIGATING OF PROTECTIVE EFFECTS OF NURR1 AND GDNF ON DOPAMINERGIC NEURAL CELL LINE SH-SY5Y AGAINST NEURO-INFLAMMATION AND TOXICITY OF 6-OHDA. JOURNAL OF CELL & TISSUE[Internet]. 2017;8(1 ):87-108. Available from: https://sid.ir/paper/189131/en

IEEE: Copy

M. RASOOLNEZHAD, M. GARDANEH, and F. SABOUNI, “INVESTIGATING OF PROTECTIVE EFFECTS OF NURR1 AND GDNF ON DOPAMINERGIC NEURAL CELL LINE SH-SY5Y AGAINST NEURO-INFLAMMATION AND TOXICITY OF 6-OHDA,” JOURNAL OF CELL & TISSUE, vol. 8, no. 1 , pp. 87–108, 2017, [Online]. Available: https://sid.ir/paper/189131/en

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