NIFH GENE STUDY OF MESORHIZOBIUM CICERO ISOLATED FROM IRAN

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MOAZEMIAN E.; KARGAR MOHAMMAD; ASGHARZADEH A.; HOSEINI MAZINANI S.M.

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Title

NIFH GENE STUDY OF MESORHIZOBIUM CICERO ISOLATED FROM IRAN

Author(s)

MOAZEMIAN E. | KARGAR MOHAMMAD | ASGHARZADEH A. | HOSEINI MAZINANI S.M. | Issue Writer Certificate

Keywords

MESORHIZOBIUM CICERIQ3

NIFHDK OPERONQ3

NITROGEN FIXATIONQ2

CICER ARIETINUMQ3

Abstract

Background and objectives: CICER ARIETINUM is the world`s third ranking and Iran`s first ranking legume in terms of amount of production. MESORHIZOBIUM CICERI is the most important bacteria that has symbiosis with this plant and fixes nitrogen for this legume. The symbiotic Rhizobium of this plant is quite effective in fixing nitrogen molecules. Therefore the study of this phenomenon is very important. Atmospheric NITROGEN FIXATION requires nitrogenase enzyme. nifH, nifD and nifK genes encode three polypeptides of the nitrogenase complex. Material and methods: After isolation of bacteria and culture it, total DNA was isolated by using a standard protocol and it was used as a template. The nifH gene was amplified from genomic DNA by PCR method with appropriate primers.Results: Using two sets of primers nifH1/nifH2 and nifH4/nifD1, amplified products of the expected size were obtained on DNA extracted from M. ciceri. The amplified products obtained with the nifH gene was purified and sequenced. The nucleotide sequences of nifH gene of M.ciceri was performed.Conclusion: 90% homology was shown with the nifH gene in M.loti. This gene has also shown high homology with nifH gene in other Rhizobium species.

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APA: Copy

MOAZEMIAN, E., KARGAR, MOHAMMAD, ASGHARZADEH, A., & HOSEINI MAZINANI, S.M.. (2009). NIFH GENE STUDY OF MESORHIZOBIUM CICERO ISOLATED FROM IRAN. JOURNAL OF MICROBIAL WORLD, 2(1), 12-18. SID. https://sid.ir/paper/189159/en

Vancouver: Copy

MOAZEMIAN E., KARGAR MOHAMMAD, ASGHARZADEH A., HOSEINI MAZINANI S.M.. NIFH GENE STUDY OF MESORHIZOBIUM CICERO ISOLATED FROM IRAN. JOURNAL OF MICROBIAL WORLD[Internet]. 2009;2(1):12-18. Available from: https://sid.ir/paper/189159/en

IEEE: Copy

E. MOAZEMIAN, MOHAMMAD KARGAR, A. ASGHARZADEH, and S.M. HOSEINI MAZINANI, “NIFH GENE STUDY OF MESORHIZOBIUM CICERO ISOLATED FROM IRAN,” JOURNAL OF MICROBIAL WORLD, vol. 2, no. 1, pp. 12–18, 2009, [Online]. Available: https://sid.ir/paper/189159/en

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