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Information Journal Paper

Title

ISOLATION, MOLECULAR IDENTIFICATION AND PRODUCTION INVESTIGATION OF BACILLUS-PRODUCING L-ASPARAGINASE FROM JIROFT MICROFLORA

Pages

  247-256

Abstract

 Background & Objectives: Cancer cells are not capable of producing L-asparagine, and mainly are depended to the L-asparagine from the circulating plasma pools. L-ASPARAGINASE (L-asparagine amidohydrolase EC 3.5.1.1) catalysis the conversion of L-asparagine to L-aspartate and ammonium. L-ASPARAGINASE is the first enzyme with anti-leukemic activity to be intensively studied in human beings. Isolation and identification of L-ASPARAGINASE producing bacteria from Jiroft microflora was the aim of this study.Materials & Methods: In this sectional study, soil and water samples were collected from citrus orchards and waste of milk factory in Jiroft, Kerman province. The bacterial-producing L-ASPARAGINASE was screened on agar medium supplied with L-asparagine and phenol red indicator dye. L-ASPARAGINASE activity was detected on the basis of pink color around the colony. The best strains were finally identified using PCR method.Results: 16S rRNA sequencing results showed that the best isolate-producing L-ASPARAGINASE belonged to the BACILLUS genus. DJK23 strain produced the maximum L-ASPARAGINASE, approximately 165 U/ml. The our analysis showed that L-ASPARAGINASE was active in a pH rang of 5 to 8, with maximal enzyme activity at pH 7. It is mentioned that this enzyme retains 36% of its initial activity at pH 5.Conclusion: Although L-ASPARAGINASE from bacteria has been extensively characterized, a similar attention has not been paid to BACILLUS. These results showed that BACILLUS L-ASPARAGINASE has desirable activity in the presence of neutral and acidic pH, which can be considered as a therapeutic and industrial enzyme.

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    APA: Copy

    BADOEI DALFARD, ARASTOO, KARAMI, ZAHRA, & RAMEZANI POUR, NARJES. (2016). ISOLATION, MOLECULAR IDENTIFICATION AND PRODUCTION INVESTIGATION OF BACILLUS-PRODUCING L-ASPARAGINASE FROM JIROFT MICROFLORA. JOURNAL OF MICROBIAL WORLD, 9(3 (28)), 247-256. SID. https://sid.ir/paper/189267/en

    Vancouver: Copy

    BADOEI DALFARD ARASTOO, KARAMI ZAHRA, RAMEZANI POUR NARJES. ISOLATION, MOLECULAR IDENTIFICATION AND PRODUCTION INVESTIGATION OF BACILLUS-PRODUCING L-ASPARAGINASE FROM JIROFT MICROFLORA. JOURNAL OF MICROBIAL WORLD[Internet]. 2016;9(3 (28)):247-256. Available from: https://sid.ir/paper/189267/en

    IEEE: Copy

    ARASTOO BADOEI DALFARD, ZAHRA KARAMI, and NARJES RAMEZANI POUR, “ISOLATION, MOLECULAR IDENTIFICATION AND PRODUCTION INVESTIGATION OF BACILLUS-PRODUCING L-ASPARAGINASE FROM JIROFT MICROFLORA,” JOURNAL OF MICROBIAL WORLD, vol. 9, no. 3 (28), pp. 247–256, 2016, [Online]. Available: https://sid.ir/paper/189267/en

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