SCREENING OF ESHERICHIA COLI STRAINS FOR ASPARAGINASE II PRODUCTION

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GHANE M.; BAMBAEI B.; GHANE MOZHGAN

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Paper Information

Journal: BIOLOGICAL SCIENCES (DANISH-I ZISTI-I IRAN) Year:2009 | Volume:3 | Issue:4 Page(s): 47-54

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Title

SCREENING OF ESHERICHIA COLI STRAINS FOR ASPARAGINASE II PRODUCTION

Author(s)

GHANE M. | BAMBAEI B. | GHANE M.ZHGAN | Issue Writer Certificate

Keywords

ASPARAGINASE IIQ3

ENZYME ASSAYQ2

E. COLIQ2

ANSBQ3

Abstract

L-ASPARAGINASE II from Echerchia coli has been supplied and employed in the acute leukemia. In this investigation, the various strains of E. COLI including LAB, K12, DH5a and TOPTEN were tested for ANSB gene by PCR reaction. The strains with ANSB gene were cultured and as the absorbance at 600 nm reached to 1, the expression was induced by anaerobic induction. After induction, cells were harvested and the supernatant and periplasmic content of cells were tested for asparaginase activity.Asparaginase activity was measured by determining the rate of amonia formation. Screening of E. COLI strains by PCR reaction revealed the ANSB gene in LAB, K12 and DH5a strains of E. COLI. Neither periplasmic nor supernatant of these strains had asparaginase activity aerobically. The amonia formation in all strains was almost the same anaerobically and about 0.01 mmol (3.6 unit/ml). The maximum enzyme production was at induction duration of 1 hour in E. COLI LAB and DH5a, and 40 minutes in E. COLI K12. The enzyme production was decreased 3 hours after anaerobic induction (2-2.5unit/ml).

Cites

ISOLATION AND MOLECULAR IDENTIFICATION OF BACILLUS SPECIES THAT PRODUCE EXTRACELLULAR L ASPARAGINASE, AN ANTI-CANCER ENZYME, FROM SOILS

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APA: Copy

GHANE, M., BAMBAEI, B., & GHANE, MOZHGAN. (2009). SCREENING OF ESHERICHIA COLI STRAINS FOR ASPARAGINASE II PRODUCTION. BIOLOGICAL SCIENCES (DANISH-I ZISTI-I IRAN), 3(4), 47-54. SID. https://sid.ir/paper/183740/en

Vancouver: Copy

GHANE M., BAMBAEI B., GHANE MOZHGAN. SCREENING OF ESHERICHIA COLI STRAINS FOR ASPARAGINASE II PRODUCTION. BIOLOGICAL SCIENCES (DANISH-I ZISTI-I IRAN)[Internet]. 2009;3(4):47-54. Available from: https://sid.ir/paper/183740/en

IEEE: Copy

M. GHANE, B. BAMBAEI, and MOZHGAN GHANE, “SCREENING OF ESHERICHIA COLI STRAINS FOR ASPARAGINASE II PRODUCTION,” BIOLOGICAL SCIENCES (DANISH-I ZISTI-I IRAN), vol. 3, no. 4, pp. 47–54, 2009, [Online]. Available: https://sid.ir/paper/183740/en

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