EXTRACTION AND CLONING OF INTERLEUKINE- 2 GENE FROM THE IRANIAN CHICKEN

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AMINI HAMIDEH; HOSEINI SEYED DAVOOD; NOWROOZI JAMILEH; SHAHBAZZADEH DELAVAR

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Journal: JOURNAL OF MICROBIAL WORLD Year:2010 | Volume:3 | Issue:1 (6) Page(s): 16-23

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Title

EXTRACTION AND CLONING OF INTERLEUKINE- 2 GENE FROM THE IRANIAN CHICKEN

Author(s)

AMINI HAMIDEH | HOSEINI SEYED DAVOOD | NOWROOZI JAMILEH | SHAHBAZZADEH DELAVAR | Issue Writer Certificate

Keywords

CHICKEN IL-2Q3

RECOMBINANT VACCINEQ2

CYTOKINEQ2

ADJUVANTQ2

CLONINGQ2

Abstract

Background and Objectives: Nowadays, the immune CYTOKINEs, such as the CHICKEN IL-2 (chIL-2) gene are incorporated into vaccination regimens used by the poultry industry. The CYTOKINEs can potentially enhance the efficiency of vaccines according to increase T lymphocyte proliferation, B lymphocyte development and natural killer cell (NK) activation. The aim of this study was extraction and CLONING of Iranian chicken interleukine-2 (chIL-2).Materials and Methods: First of all, in order to extract chIL-2 gene, total cell RNAs were isolated by culturing the harvested splenocytes and using Trizol reagent. The chIL-2 specific mRNA was converted into cDNA using reverse Transcriptase (RT) and specific designed primers. Then, The PCR product was ligated into the PTZ57R/T plasmid (TA-cloning kit) and was transformed into the competent Top10 E. coli using heat shock method.Results: A unique band of 668-bp was obtained after RT-PCR amplification. The results of restriction enzyme, colony PCR from transformed colonies and also gene sequencing confirmed the existence of desired gene in transformants bacteria.Conclusion: For the first time in Iran we could extract and clone the chIL-2 gene in bacteria and gene alignment. A direct sequencing test showed 99% similarity between this gene and the established data in NCBI.

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APA: Copy

AMINI, HAMIDEH, HOSEINI, SEYED DAVOOD, NOWROOZI, JAMILEH, & SHAHBAZZADEH, DELAVAR. (2010). EXTRACTION AND CLONING OF INTERLEUKINE- 2 GENE FROM THE IRANIAN CHICKEN. JOURNAL OF MICROBIAL WORLD, 3(1 (6)), 16-23. SID. https://sid.ir/paper/189282/en

Vancouver: Copy

AMINI HAMIDEH, HOSEINI SEYED DAVOOD, NOWROOZI JAMILEH, SHAHBAZZADEH DELAVAR. EXTRACTION AND CLONING OF INTERLEUKINE- 2 GENE FROM THE IRANIAN CHICKEN. JOURNAL OF MICROBIAL WORLD[Internet]. 2010;3(1 (6)):16-23. Available from: https://sid.ir/paper/189282/en

IEEE: Copy

HAMIDEH AMINI, SEYED DAVOOD HOSEINI, JAMILEH NOWROOZI, and DELAVAR SHAHBAZZADEH, “EXTRACTION AND CLONING OF INTERLEUKINE- 2 GENE FROM THE IRANIAN CHICKEN,” JOURNAL OF MICROBIAL WORLD, vol. 3, no. 1 (6), pp. 16–23, 2010, [Online]. Available: https://sid.ir/paper/189282/en

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