COLONING AND EXPRESSION OF FLIC AS A VACCINE CANDIDATE AGAINST TYPHOID

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ADELI ZAHRA; Farahani Najmeh; MOSAVI SEYED AHMAD; SEHRI SEYED YAGHOOB; MORADI MOGARMOON HEKMAT ALLAH; GHEIBI HAYAT SEYED MOHAMMAD

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Journal: Studies in Medical Sciences Year:2016 | Volume:27 | Issue:8 Page(s): 683-691

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Title

COLONING AND EXPRESSION OF FLIC AS A VACCINE CANDIDATE AGAINST TYPHOID

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Keywords

SALMONELLAQ3

RECOMBINANT VACCINEQ2

FLICQ1

CLONING AND EXPRESSIONQ1

Abstract

Background & Aims: SALMONELLA enterica is a zoonotic pathogen causing typhoid fever in humans and animals. It is an important cause of food borne infections in humans throughout the world. A recent study estimated approximately 22 million cases of typhoid each year with at least 200, 000 deaths. FLIC encoding flagellin plays a role in pathogenesis and is an important antigen in vaccination. In this study, recombinant protein FLIC as a candidate vaccine against salmonellosis was produced and purified.Materials & Methods: The protein sequence of FLIC was obtained from NCBI database. For high level expression of protein, the gene was synthesized with codon bias of E. coli.; synthetic gene in pUC57 was sub coloned into pET32 expression vector and then transferred into E. coli BL21DE3. The recombinant protein was express by IPTG induction. For high expression, three parameters including IPTG concentration, time and temperature of induction were optimized. The recombinant protein was purified by ion exchange chromatography with His-Tag. The purified protein was confirmed by western blotting.Results: Recombinant vector was approved by PCR and restriction analysis. SDS-PAGE showed a 51 kDa protein with proper purity and western blotting with his-tag antibody confirmed the intend protein.Conclusion: The use of RECOMBINANT VACCINEs is highly regarded, since one of the major virulence factors of SALMONELLA gene was cloned and the protein was produced and purified in this study. This protein can be used in candidate vaccine against typhoid.

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APA: Copy

ADELI, ZAHRA, Farahani, Najmeh, MOSAVI, SEYED AHMAD, SEHRI, SEYED YAGHOOB, MORADI MOGARMOON, HEKMAT ALLAH, & GHEIBI HAYAT, SEYED MOHAMMAD. (2016). COLONING AND EXPRESSION OF FLIC AS A VACCINE CANDIDATE AGAINST TYPHOID. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), 27(8), 683-691. SID. https://sid.ir/paper/64175/en

Vancouver: Copy

ADELI ZAHRA, Farahani Najmeh, MOSAVI SEYED AHMAD, SEHRI SEYED YAGHOOB, MORADI MOGARMOON HEKMAT ALLAH, GHEIBI HAYAT SEYED MOHAMMAD. COLONING AND EXPRESSION OF FLIC AS A VACCINE CANDIDATE AGAINST TYPHOID. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES)[Internet]. 2016;27(8):683-691. Available from: https://sid.ir/paper/64175/en

IEEE: Copy

ZAHRA ADELI, Najmeh Farahani, SEYED AHMAD MOSAVI, SEYED YAGHOOB SEHRI, HEKMAT ALLAH MORADI MOGARMOON, and SEYED MOHAMMAD GHEIBI HAYAT, “COLONING AND EXPRESSION OF FLIC AS A VACCINE CANDIDATE AGAINST TYPHOID,” STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), vol. 27, no. 8, pp. 683–691, 2016, [Online]. Available: https://sid.ir/paper/64175/en

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