IMMUNIZATION OF PSEUDOMONAS AERUGINOSA ALGINATE-CONJUGATED TO DIPHTERIA TOXOID IN MICE

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JABERI GHAZALEH; SHAPOURI REZA; KARIMINIK ASHRAF

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Journal: JOURNAL OF MICROBIAL WORLD Year:2013 | Volume:6 | Issue:2 (15) Page(s): 138-147

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Information Journal Paper

Title

IMMUNIZATION OF PSEUDOMONAS AERUGINOSA ALGINATE-CONJUGATED TO DIPHTERIA TOXOID IN MICE

Author(s)

JABERI GHAZALEH | SHAPOURI REZA | KARIMINIK ASHRAF | Issue Writer Certificate

Keywords

PSEUDOMONAS AEROGINOSAQ2

ALGINATEQ2

DIPHTHERIA TOXOIDQ2

CONJUGATE VACCINEQ2

ELISAQ2

Abstract

Background and Objectives: Pseudomonas aeruginosa is one of the most important infectious agent in medicine. These bacteria cause several infections especially in the patient with high degrees of burns and in immunodeficiency cases. The purpose of this study was providing conjugated antigen component that can induct production of antibody and memory immune in mouse model against Pseudomonas aeruginosa.Materials and Methods: After culturing P. aeruginosa strain PAO1 and extraction of ALGINATE, Diphteria toxoid, ADH and EDAC were added as protein carrier, spacer and linker, respectively to the ALGINATE. After passing the ALGINATE DIPHTHERIA TOXOID conjugated (ALG-DT) through chromatography column (CL-2B), its quality was checked to get the quality control label. The prepared antigens were intraperitoneally injected to BALB/c mice (10mg/ml/mouse) for three times once each two weeks. Blood sampling was performed after two weeks of each injection and the levels of serum IgG, IgA and IgM were measured by ELISA.Results: The titer of serum antibodies of vaccinated group with ALG-DT increased significantly after each injection. The levels of serum IgG, IgM and IgA against ALGINATE in vaccinated group was significantly more than the control groups, and after third injection reached to 3.5, 1.7 and 1.2 times increases, respectively.Conclusion: The increases in the levels of antibodies in vaccinated groups are an indicator of activation of T-cells and memory cells. As a result, the conjugated ALGINATE-diphtheria toxoid can be appropriate candidates for production of vaccine.

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APA: Copy

JABERI, GHAZALEH, SHAPOURI, REZA, & KARIMINIK, ASHRAF. (2013). IMMUNIZATION OF PSEUDOMONAS AERUGINOSA ALGINATE-CONJUGATED TO DIPHTERIA TOXOID IN MICE. JOURNAL OF MICROBIAL WORLD, 6(2 (15)), 138-147. SID. https://sid.ir/paper/189295/en

Vancouver: Copy

JABERI GHAZALEH, SHAPOURI REZA, KARIMINIK ASHRAF. IMMUNIZATION OF PSEUDOMONAS AERUGINOSA ALGINATE-CONJUGATED TO DIPHTERIA TOXOID IN MICE. JOURNAL OF MICROBIAL WORLD[Internet]. 2013;6(2 (15)):138-147. Available from: https://sid.ir/paper/189295/en

IEEE: Copy

GHAZALEH JABERI, REZA SHAPOURI, and ASHRAF KARIMINIK, “IMMUNIZATION OF PSEUDOMONAS AERUGINOSA ALGINATE-CONJUGATED TO DIPHTERIA TOXOID IN MICE,” JOURNAL OF MICROBIAL WORLD, vol. 6, no. 2 (15), pp. 138–147, 2013, [Online]. Available: https://sid.ir/paper/189295/en

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