INVESTIGATION OF GENETIC DIVERSITY OF MYCOBACTERIUM FORTUITUM CLINICAL ISOLATED BY RAPD-PCR

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SHEINI MEHRABZADEH RASA; KHOSRAVI AZARDOKHT; HASHEMI ABDOLRAZZAGH; JAMALI HOOSHANGH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF MICROBIAL WORLD Year:2014 | Volume:6 | Issue:4 (17) Page(s): 281-289

Download Full-Text

Persian Verion

View:

622

Download:

Cites:

Information Journal Paper

Title

INVESTIGATION OF GENETIC DIVERSITY OF MYCOBACTERIUM FORTUITUM CLINICAL ISOLATED BY RAPD-PCR

Author(s)

SHEINI MEHRABZADEH RASA | KHOSRAVI AZARDOKHT | HASHEMI ABDOLRAZZAGH | JAMALI HOOSHANGH | Issue Writer Certificate

Keywords

MYCOBACTERIUM FORTUITUMQ2

RAPD-PCRQ2

MOLECULAR TYPINGQ2

Abstract

Background and Objectives: Non-tuberculosis Mycobacteria (NTM) are important due to increase in the rate of clinical outbreaks and emerging antibiotic resistance. MYCOBACTERIUM FORTUITUM is the most common NTMs in Iran. This study was aimed to molecular detection and genetic diversity of clinical isolates of M. foruitum in Iran using RAPD-PCR method.Materials and Methods: This cross-sectional study was carried out on 81 isolates of NTM isolated from various clinical samples. The primary identification of M. fortuitum was performed based on Acid Fast staining and routine biochemical tests. After confirmation of the isolates based on PCR, the PCR products were digested with AvaII, HphI and HpaII. The MOLECULAR TYPING of the M. fortuitum isolates were performed using PAPD analysis.Results: Out of 81 tested NTM, 36 strains were confirmed as M. fortuitum. Based on RAPD primers, these isolates were classified into 10 main clusters. Most of the isolates were distributed into RAPD types of 1 (9 members, 25%), 2 (8 members, 22.2%) and 6 (6 members, 16.6%). In RAPD analysis, the major fragments were 300 bp (94.4%), followed by fragment 1000 bp (66.6%).Conclusion: The results showed high discriminatory ability of RAPD–PCR method. This analysis is able to sufficiently discriminate the genotypic diversity and to prepare epidemiologic information of the M. fortuitum isolates. RAPD techniques is simple, rapid, inexpensive, and is less complicated than most of other MOLECULAR TYPING methods.

Cites

No record.

References

No record.

Cite

APA: Copy

SHEINI MEHRABZADEH, RASA, KHOSRAVI, AZARDOKHT, HASHEMI, ABDOLRAZZAGH, & JAMALI, HOOSHANGH. (2014). INVESTIGATION OF GENETIC DIVERSITY OF MYCOBACTERIUM FORTUITUM CLINICAL ISOLATED BY RAPD-PCR. JOURNAL OF MICROBIAL WORLD, 6(4 (17)), 281-289. SID. https://sid.ir/paper/189305/en

Vancouver: Copy

SHEINI MEHRABZADEH RASA, KHOSRAVI AZARDOKHT, HASHEMI ABDOLRAZZAGH, JAMALI HOOSHANGH. INVESTIGATION OF GENETIC DIVERSITY OF MYCOBACTERIUM FORTUITUM CLINICAL ISOLATED BY RAPD-PCR. JOURNAL OF MICROBIAL WORLD[Internet]. 2014;6(4 (17)):281-289. Available from: https://sid.ir/paper/189305/en

IEEE: Copy

RASA SHEINI MEHRABZADEH, AZARDOKHT KHOSRAVI, ABDOLRAZZAGH HASHEMI, and HOOSHANGH JAMALI, “INVESTIGATION OF GENETIC DIVERSITY OF MYCOBACTERIUM FORTUITUM CLINICAL ISOLATED BY RAPD-PCR,” JOURNAL OF MICROBIAL WORLD, vol. 6, no. 4 (17), pp. 281–289, 2014, [Online]. Available: https://sid.ir/paper/189305/en

Related Journal Papers