CONSTRUCTION OF GLUCANASE GENE OF BACILLUS SUBTILIS IN ESCHERICHIA COLI

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SHAVALI MARZIEH; DOOSTI ABBAS

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Journal: JOURNAL OF MICROBIAL WORLD Year:2016 | Volume:8 | Issue:4 (25) Page(s): 264-270

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Title

CONSTRUCTION OF GLUCANASE GENE OF BACILLUS SUBTILIS IN ESCHERICHIA COLI

Author(s)

SHAVALI MARZIEH | DOOSTI ABBAS | Issue Writer Certificate

Keywords

β-GLUCANASEQ2

BACILLUS SUBTILISQ2

T/A CLONINGQ2

Abstract

Background & Objectives: The grains are considered as the main food for poultries. Grains contain significant content of non- soluble starch polysaccharides (NSP). NSPs cause negative impact and incompatibility in birds. Digestion of NSP contents by b-glucanase enzyme supplement reduces the viscosity of polysaccharides in the digestive tracts, and increases their intestinal absorption. The aim of this study was to construction of glucanase gene of BACILLUS SUBTILIS in Escherichia coli.Materials & Methods: In this experimental study, the glucanase gene of B. subtilis was amplified using specific primers and polymerase chain reaction. After purification of the bands, Bgl gene was cloned by T/A CLONING technique in pGEM vector, and was transformed in E. coli. Afterward, the pGEM-bgl was transferred into E. coli Top-10 strain. The recombinant vectors were then transformed into E. coli competent cells, and the recombinants were plated on LB agar containing 100 mg/ml ampicillin. The recombinant plasmid transformed to E. coli Top10 cell and the colonies carrying plasmid were selected by PCR. The presence of glucanase construction was confirmed by enzymatic digestion.Results: The results showed that the glucanase gene was successfully cloned in E. coli. The results of cloning of 767 bp glucanase gene was confirmed by PCR assay.Conclusion: The construction of B. subtilils glucanase gene and cloning of this gene in E. coli is a strong alternative for the production of probiotic used for poultry.

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APA: Copy

SHAVALI, MARZIEH, & DOOSTI, ABBAS. (2016). CONSTRUCTION OF GLUCANASE GENE OF BACILLUS SUBTILIS IN ESCHERICHIA COLI. JOURNAL OF MICROBIAL WORLD, 8(4 (25)), 264-270. SID. https://sid.ir/paper/189328/en

Vancouver: Copy

SHAVALI MARZIEH, DOOSTI ABBAS. CONSTRUCTION OF GLUCANASE GENE OF BACILLUS SUBTILIS IN ESCHERICHIA COLI. JOURNAL OF MICROBIAL WORLD[Internet]. 2016;8(4 (25)):264-270. Available from: https://sid.ir/paper/189328/en

IEEE: Copy

MARZIEH SHAVALI, and ABBAS DOOSTI, “CONSTRUCTION OF GLUCANASE GENE OF BACILLUS SUBTILIS IN ESCHERICHIA COLI,” JOURNAL OF MICROBIAL WORLD, vol. 8, no. 4 (25), pp. 264–270, 2016, [Online]. Available: https://sid.ir/paper/189328/en

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