STUDY OF PHYSICOCHEMICAL FACTORS ON DIPICOLINIC ACID FORMATION BY BACILLUS CEREUS PTCC1015

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NIKSIRAT SABER; FAEZI GHASEMI MOHAMMAD; ISASAZADH KHOSRO; KHOSH KHOLGH PAHLAVIANI MOHAMMAD REZA

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Journal: JOURNAL OF MICROBIAL WORLD Year:2012 | Volume:4 | Issue:3-4 (11) Page(s): 93-100

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Title

STUDY OF PHYSICOCHEMICAL FACTORS ON DIPICOLINIC ACID FORMATION BY BACILLUS CEREUS PTCC1015

Author(s)

NIKSIRAT SABER | FAEZI GHASEMI MOHAMMAD | ISASAZADH KHOSRO | KHOSH KHOLGH PAHLAVIANI MOHAMMAD REZA | Issue Writer Certificate

Keywords

DPAQ2

COLORIMETRICQ2

SPOREQ2

BACILLUS CEREUSQ2

Abstract

Background and Objective: Dipicolinic acid (pyridne 2, 6 dicarboxylic acid) is a major component of bacterial SPORE and is unique in that it has been found only in bacterial endospore. This study was aim to evaluate the environmental stress on DPA production in BACILLUS CEREUS PTCC1015. Materials and Methods: In this experimental study the effects of environmental stresses including temperature, ethanol, NaCl and pH on the production of DPA by BACILLUS CEREUS PTCC 1015 were evaluated by COLORIMETRIC assay (the color complex formed by interaction of ferrous iron with dipicolinic acid) and comparison with standard curves.Results: The results showed that the amount of DPA in B. cereus decreased upon increase in acidic condition and salt concentration in the medium. DPA formation increased at alkaline pH 8-10. DPA formation decreased in the medium containing 5-55% of ethanol. DPA formation was higher in optimum temperature at 30oC in comparison to the reference strains. The cell concentration decreased at 90oC and level of DPA detected in this stage.Conclusion: In stressful condition, DPA formation significantly reduced and these properties can be used in food and dairy industries to control SPORE production of BACILLUS CEREUS.

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APA: Copy

NIKSIRAT, SABER, FAEZI GHASEMI, MOHAMMAD, ISASAZADH, KHOSRO, & KHOSH KHOLGH PAHLAVIANI, MOHAMMAD REZA. (2012). STUDY OF PHYSICOCHEMICAL FACTORS ON DIPICOLINIC ACID FORMATION BY BACILLUS CEREUS PTCC1015. JOURNAL OF MICROBIAL WORLD, 4(3-4 (11)), 93-100. SID. https://sid.ir/paper/189393/en

Vancouver: Copy

NIKSIRAT SABER, FAEZI GHASEMI MOHAMMAD, ISASAZADH KHOSRO, KHOSH KHOLGH PAHLAVIANI MOHAMMAD REZA. STUDY OF PHYSICOCHEMICAL FACTORS ON DIPICOLINIC ACID FORMATION BY BACILLUS CEREUS PTCC1015. JOURNAL OF MICROBIAL WORLD[Internet]. 2012;4(3-4 (11)):93-100. Available from: https://sid.ir/paper/189393/en

IEEE: Copy

SABER NIKSIRAT, MOHAMMAD FAEZI GHASEMI, KHOSRO ISASAZADH, and MOHAMMAD REZA KHOSH KHOLGH PAHLAVIANI, “STUDY OF PHYSICOCHEMICAL FACTORS ON DIPICOLINIC ACID FORMATION BY BACILLUS CEREUS PTCC1015,” JOURNAL OF MICROBIAL WORLD, vol. 4, no. 3-4 (11), pp. 93–100, 2012, [Online]. Available: https://sid.ir/paper/189393/en

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