CLONING OF CORYNEBACTERIUM DIPHTHERIAE TOXIN GENE

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MOHAMMADI N.; PAKZAD P.; BANDEHPOUR M.; YARIAN M.; YAZDANFAR M.; KAZEMI B.

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Journal: JOURNAL OF MICROBIOLOGY KNOWLEDGE Year:2010 | Volume:2 | Issue:6 Page(s): 37-42

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Title

CLONING OF CORYNEBACTERIUM DIPHTHERIAE TOXIN GENE

Author(s)

Keywords

DIPHTHERIAQ3

CORYNEBACTERIUM DIPHTHERIAE TOXIN GENEQ3

RECOMBINANT PROTEINQ3

Abstract

The aim of this study was cloning DIPHTHERIA toxin gene to produce RECOMBINANT PROTEIN and application in next investigations. PCR was carried out using dt gene specific primers. This gene was cloned in pTZ57R/T vector and sub cloned into pETDuet-1 expression vector. DIPHTHERIA toxin gene was amplified successfully and cloned in pTZ57R. Recombinant plasmid was digested by restriction enzymes and released fragment (DIPHTHERIA toxin gene) sub cloned in pETDuet-1 expression vector. In this study, the DIPHTHERIA toxin gene was cloned in expression vector and confirmed, and then it became ready to make RECOMBINANT PROTEIN. It can be used in next investigations.

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APA: Copy

MOHAMMADI, N., PAKZAD, P., BANDEHPOUR, M., YARIAN, M., YAZDANFAR, M., & KAZEMI, B.. (2010). CLONING OF CORYNEBACTERIUM DIPHTHERIAE TOXIN GENE. JOURNAL OF MICROBIOLOGY KNOWLEDGE, 2(6), 37-42. SID. https://sid.ir/paper/193204/en

Vancouver: Copy

MOHAMMADI N., PAKZAD P., BANDEHPOUR M., YARIAN M., YAZDANFAR M., KAZEMI B.. CLONING OF CORYNEBACTERIUM DIPHTHERIAE TOXIN GENE. JOURNAL OF MICROBIOLOGY KNOWLEDGE[Internet]. 2010;2(6):37-42. Available from: https://sid.ir/paper/193204/en

IEEE: Copy

N. MOHAMMADI, P. PAKZAD, M. BANDEHPOUR, M. YARIAN, M. YAZDANFAR, and B. KAZEMI, “CLONING OF CORYNEBACTERIUM DIPHTHERIAE TOXIN GENE,” JOURNAL OF MICROBIOLOGY KNOWLEDGE, vol. 2, no. 6, pp. 37–42, 2010, [Online]. Available: https://sid.ir/paper/193204/en

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