Comparison of the Diphtheria Toxin Separation Methods on Standard Vaccine Strain Using Separator and Filter Presses to Improve the Quality & Quantity of the Final Product

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Faramarzi Ardeshir; NOOFELI MOJTABA; TOFIGHI AZADEH; SHAHCHERAGHI FERESHTEH

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Journal: Jundishapur Scientific Medical Journal Year:2019 | Volume:18 | Issue:5 Page(s): 459-470

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Information Journal Paper

Title

Comparison of the Diphtheria Toxin Separation Methods on Standard Vaccine Strain Using Separator and Filter Presses to Improve the Quality & Quantity of the Final Product

Author(s)

Faramarzi Ardeshir | NOOFELI MOJTABA | TOFIGHI AZADEH | SHAHCHERAGHI FERESHTEH | Issue Writer Certificate

Keywords

Corynebacterium DiphtheriaQ1

VaccineQ2

Toxin SeparationQ1

SeparatorQ1

Filter PressQ1

Abstract

Background & Aim: The aim of this research was based on increasing of the quantity, quality and effectiveness of this Vaccine, protection of personnel and cost-benefit of production. In this regard two diphtheria Toxin Separation methods (Separator and Filter Press) were compared. Methods: Corynebacterium Diphtheria (PW-8/CN-2000) was cultivated in 350L fermentor and half of the cultivation toxin clarified by Seitz Filter Press method and diatomaceous earth as filter aid and the rest was clarified by Separator. Three sterile samples (50 mL) of each three produced batches were assessed by minimum lethal dose (MLD), specific toxicity test (STT), toxin flocculation titer (Lf, Kf – Ramon flocculation test) and SDS– PAGE. Other parameters like time consumption for toxin clarification with specific volume, quantity, product clarification, volume and clarification of product in two methods compared. Results: The results confirmed that the Separator clarification method was more advantageous to Filter Press. Also, volume and transparency of the final product after clarification by Separator were better than Filter Press method. In addition, the separation time and costs used in the Separator method were much less than Filter Press method. Conclusion: In conclusion, use of Separator clarification method was suitable alternative to current Filter Press method in terms of quantity and quality of diphtheria Vaccine production.

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APA: Copy

Faramarzi, Ardeshir, NOOFELI, MOJTABA, TOFIGHI, AZADEH, & SHAHCHERAGHI, FERESHTEH. (2019). Comparison of the Diphtheria Toxin Separation Methods on Standard Vaccine Strain Using Separator and Filter Presses to Improve the Quality & Quantity of the Final Product. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, 18(5 ), 459-470. SID. https://sid.ir/paper/365419/en

Vancouver: Copy

Faramarzi Ardeshir, NOOFELI MOJTABA, TOFIGHI AZADEH, SHAHCHERAGHI FERESHTEH. Comparison of the Diphtheria Toxin Separation Methods on Standard Vaccine Strain Using Separator and Filter Presses to Improve the Quality & Quantity of the Final Product. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL[Internet]. 2019;18(5 ):459-470. Available from: https://sid.ir/paper/365419/en

IEEE: Copy

Ardeshir Faramarzi, MOJTABA NOOFELI, AZADEH TOFIGHI, and FERESHTEH SHAHCHERAGHI, “Comparison of the Diphtheria Toxin Separation Methods on Standard Vaccine Strain Using Separator and Filter Presses to Improve the Quality & Quantity of the Final Product,” JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, vol. 18, no. 5 , pp. 459–470, 2019, [Online]. Available: https://sid.ir/paper/365419/en

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