STUDY OF EFFECTIVENESS OF THREE DIFFERENT LYSIS BUFFER IN DNA EXTRACTION OF LEPTOSPIRA

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MORADI BIDHENDI S.; ASHOORZADEH R.; KHAKI P.

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Journal: JOURNAL OF MICROBIOLOGY KNOWLEDGE Year:2010 | Volume:2 | Issue:6 Page(s): 7-12

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Title

STUDY OF EFFECTIVENESS OF THREE DIFFERENT LYSIS BUFFER IN DNA EXTRACTION OF LEPTOSPIRA

Author(s)

MORADI BIDHENDI S. | ASHOORZADEH R. | KHAKI P. | Issue Writer Certificate

Keywords

LEPTOSPIRAQ3

LEPTOSPIROSISQ3

LYSIS BUFFERQ3

DNA EXTRACTIONQ3

Abstract

Leptospirosis is a zoonosis disease that caused by pathogenic serovars of LEPTOSPIRA which spreads in tropical countries. Detection of LEPTOSPIRA from the sample by the culture is time consuming and molecular methods like PCR are rapid and suitable method. The aim of this study is prepare the best lyses buffer for using in LEPTOSPIRA DNA EXTRACTION. The pathogenic serovars of LEPTOSPIRA cultured on EMJH medium and pellet of the bacteria were collected. Three LYSIS BUFFERs, Lep1, Lep2 and Lep3 were made for the extraction of LEPTOSPIRAl DNA by using phenol- chloroform-isoamyl alcohol. The extracted DNA was analyzed by Spectrophotometer, agarose gel electrophoresis and PCR. The results showed that Lep1 LYSIS BUFFER has the best quality of extracted DNA. Moreover, the results of PCR showed that extracted DNA with Lep1 LYSIS BUFFER was more suitable for PCR.

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APA: Copy

MORADI BIDHENDI, S., ASHOORZADEH, R., & KHAKI, P.. (2010). STUDY OF EFFECTIVENESS OF THREE DIFFERENT LYSIS BUFFER IN DNA EXTRACTION OF LEPTOSPIRA. JOURNAL OF MICROBIOLOGY KNOWLEDGE, 2(6), 7-12. SID. https://sid.ir/paper/193209/en

Vancouver: Copy

MORADI BIDHENDI S., ASHOORZADEH R., KHAKI P.. STUDY OF EFFECTIVENESS OF THREE DIFFERENT LYSIS BUFFER IN DNA EXTRACTION OF LEPTOSPIRA. JOURNAL OF MICROBIOLOGY KNOWLEDGE[Internet]. 2010;2(6):7-12. Available from: https://sid.ir/paper/193209/en

IEEE: Copy

S. MORADI BIDHENDI, R. ASHOORZADEH, and P. KHAKI, “STUDY OF EFFECTIVENESS OF THREE DIFFERENT LYSIS BUFFER IN DNA EXTRACTION OF LEPTOSPIRA,” JOURNAL OF MICROBIOLOGY KNOWLEDGE, vol. 2, no. 6, pp. 7–12, 2010, [Online]. Available: https://sid.ir/paper/193209/en

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