RAPID DETECTION OF RIFAMPIN RESISTANCE IN MYCOBACTERIUM TUBERCULOSIS IN IRANIAN PATIENTS USING A SINGLE MULTIPLEX ALLELE-SPECIFIC POLYMERASE CHAIN REACTION (PCR) ASSAY

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TAGHAVI K.; FARNIA P.; AHMADI M.; NOWROOZI J.; KAZEMPOOR M.; MASJEDI M.; VELAYATI A.

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Journal: JOURNAL OF MICROBIOLOGY KNOWLEDGE Year:2009 | Volume:1 | Issue:3 Page(s): 49-54

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Title

RAPID DETECTION OF RIFAMPIN RESISTANCE IN MYCOBACTERIUM TUBERCULOSIS IN IRANIAN PATIENTS USING A SINGLE MULTIPLEX ALLELE-SPECIFIC POLYMERASE CHAIN REACTION (PCR) ASSAY

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Keywords

MULTIDRUG RESISTANTQ4

TUBERCULOSISQ4

MULTIPLEX PCRQ4

RIFAMPINQ4

Abstract

Prompt detection of Mycobacterium TUBERCULOSIS drug resistance is essential for effective control of TUBERCULOSIS (TB). The most frequent mutations associated with RIFAMPIN resistance in Mycobacterium are the substitutions at codons 516, 526 and 531 in rpoB gene. The aim of this study was to characterize these mutations in Mycobacterium isolates from patients suspected to be infected with drug-resistant (DR) pulmonary TUBERCULOSIS (TB). 100 sample were obtained from Micobacteriulogy research center,Tehran. We developed a MAS-PCR targeting mutation in codons 516, 526, and 531 of the rpoB gene using four allele-specific primers. Drug susceptibility testing revealed that 30 (31.3%) were susceptible while 66 (68.8%) were drug resistant. 43 (44/8%) of the drug resistant isolates, were resistant to RIFAMPIN. Analysis with MULTIPLEX PCR method performed in the isolates showed that 5 (13/5%) had mutations at codon 516, 19 (51/8%) at codon 526 and 13 (35/1%) had mutations at codon 531 of rpoB gene. In conclusion, the DR profile of the isolates suggests a significant number of different DR-TB strains with a low frequency of mutation at codon 516 and high frequancy of mutation at codon 526. This method can be used on direct sputum and results can be acquired by 3-4 Days instead of 2 months.

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APA: Copy

TAGHAVI, K., FARNIA, P., AHMADI, M., NOWROOZI, J., KAZEMPOOR, M., MASJEDI, M., & VELAYATI, A.. (2009). RAPID DETECTION OF RIFAMPIN RESISTANCE IN MYCOBACTERIUM TUBERCULOSIS IN IRANIAN PATIENTS USING A SINGLE MULTIPLEX ALLELE-SPECIFIC POLYMERASE CHAIN REACTION (PCR) ASSAY. JOURNAL OF MICROBIOLOGY KNOWLEDGE, 1(3), 49-54. SID. https://sid.ir/paper/193227/en

Vancouver: Copy

TAGHAVI K., FARNIA P., AHMADI M., NOWROOZI J., KAZEMPOOR M., MASJEDI M., VELAYATI A.. RAPID DETECTION OF RIFAMPIN RESISTANCE IN MYCOBACTERIUM TUBERCULOSIS IN IRANIAN PATIENTS USING A SINGLE MULTIPLEX ALLELE-SPECIFIC POLYMERASE CHAIN REACTION (PCR) ASSAY. JOURNAL OF MICROBIOLOGY KNOWLEDGE[Internet]. 2009;1(3):49-54. Available from: https://sid.ir/paper/193227/en

IEEE: Copy

K. TAGHAVI, P. FARNIA, M. AHMADI, J. NOWROOZI, M. KAZEMPOOR, M. MASJEDI, and A. VELAYATI, “RAPID DETECTION OF RIFAMPIN RESISTANCE IN MYCOBACTERIUM TUBERCULOSIS IN IRANIAN PATIENTS USING A SINGLE MULTIPLEX ALLELE-SPECIFIC POLYMERASE CHAIN REACTION (PCR) ASSAY,” JOURNAL OF MICROBIOLOGY KNOWLEDGE, vol. 1, no. 3, pp. 49–54, 2009, [Online]. Available: https://sid.ir/paper/193227/en

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