EFFECT OF CYSTEAMINE SUPPLEMENTATION ON IN VITRO MATURATION OF BOVINE OOCYTE

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BEHESHTI R.; GHIASI GHALEH KANDI J.; MOHAMMADI ROSHANDEH A.

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Journal: JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN Year:2012 | Volume:9 (NEW) | Issue:1 (36) Page(s): 579-583

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Information Journal Paper

Title

EFFECT OF CYSTEAMINE SUPPLEMENTATION ON IN VITRO MATURATION OF BOVINE OOCYTE

Author(s)

BEHESHTI R. | GHIASI GHALEH KANDI J. | MOHAMMADI ROSHANDEH A. | Issue Writer Certificate

Keywords

BOVINE OOCYTEQ2

CYSTEAMINEQ2

IVMQ2

ANTI-OXIDANTQ1

Abstract

This study was aimed at determining the effect of CYSTEAMINE supplement during in vitro maturation of BOVINE OOCYTEs. Cumulus-oocyte complexes (COCs) from abattoir ovaries were were obtained from local abattoir (Tabriz abattoir, East Azarbaijan, Iran) shortly after slaughter and transported to the laboratory in 0.9% NaCl solution plus 100 IU/ml potassium penicillin G, and 100 mg/ml streptomycin sulfate at 30-35oC, within 2 to 4 h from slaughter. Cumulus-oocyte complexes (COCs) from abattoir ovaries were matured in vitro in Hepes-TCM 199 supplemented with 0.2 mM sodium pyruvate, 1 mg/ml 17-b-estradiol, 10% fetal calf serum (FCS), 0.5 mg/ml bFSH and 0 (control) and 0.1, 0.5 or 1 mM/ml of CYSTEAMINE for 24 h. When COCs matured in TCM 199 media with 0.1 mM/ml CYSTEAMINE, the rate of maturation were increased as compared with control group (46.66 vs 33.33%, respectively). The maturation rate of oocytes in media with 0.5 mM/ml CYSTEAMINE (56.55%) was significantly higher than control group (33.33%) (P<0.05). Also, the percentage of unmatured oocytes in 1 mM/ml treatment group lower than other groups (P<0.05). The results demonstrate that CYSTEAMINE when present during IVM, improved BOVINE OOCYTE maturation.

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APA: Copy

BEHESHTI, R., GHIASI GHALEH KANDI, J., & MOHAMMADI ROSHANDEH, A.. (2012). EFFECT OF CYSTEAMINE SUPPLEMENTATION ON IN VITRO MATURATION OF BOVINE OOCYTE. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, 9 (NEW)(1 (36)), 579-583. SID. https://sid.ir/paper/197491/en

Vancouver: Copy

BEHESHTI R., GHIASI GHALEH KANDI J., MOHAMMADI ROSHANDEH A.. EFFECT OF CYSTEAMINE SUPPLEMENTATION ON IN VITRO MATURATION OF BOVINE OOCYTE. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN[Internet]. 2012;9 (NEW)(1 (36)):579-583. Available from: https://sid.ir/paper/197491/en

IEEE: Copy

R. BEHESHTI, J. GHIASI GHALEH KANDI, and A. MOHAMMADI ROSHANDEH, “EFFECT OF CYSTEAMINE SUPPLEMENTATION ON IN VITRO MATURATION OF BOVINE OOCYTE,” JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, vol. 9 (NEW), no. 1 (36), pp. 579–583, 2012, [Online]. Available: https://sid.ir/paper/197491/en

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