A SIMPLE, FAST AND COMMERCIAL METHOD FOR TRYPSIN PURIFICATION FROM BOVINE PANCREAS

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GOUDARZI B.GH.; TALEBI M.; BAGHERIAN R.; HAJI HOSSEINI R.

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Journal: Veterinary Research & Biological Products Year:2011 | Volume:24 | Issue:1 (90) Page(s): 33-39

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Information Journal Paper

Title

A SIMPLE, FAST AND COMMERCIAL METHOD FOR TRYPSIN PURIFICATION FROM BOVINE PANCREAS

Author(s)

GOUDARZI B.GH. | TALEBI M. | BAGHERIAN R. | HAJI HOSSEINI R. | Issue Writer Certificate

Keywords

TRYPSINQ2

PURIFICATIONQ2

BOVINE PANCREASQ2

SIMPLE METHODQ2

Abstract

TRYPSIN is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. TRYPSIN has been used widely in various biotechnological processes. In this study TRYPSIN was purified using two precipitation cut, 35% and 75% as saturation percent of Ammonium sulfate solutions, TCA precipitation (2.5% W/V), pH precipitation and finally affinity chromatography. This method could be used to obtain 2.8 g purified TRYPSIN with specific activity 155 U/mg and 26% recovery from 1kg BOVINE PANCREAS.Therefore, this method is a simple, fast and commercial method for scale-up of TRYPSIN extraction from BOVINE PANCREAS.

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APA: Copy

GOUDARZI, B.GH., TALEBI, M., BAGHERIAN, R., & HAJI HOSSEINI, R.. (2011). A SIMPLE, FAST AND COMMERCIAL METHOD FOR TRYPSIN PURIFICATION FROM BOVINE PANCREAS. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), 24(1 (90)), 33-39. SID. https://sid.ir/paper/200978/en

Vancouver: Copy

GOUDARZI B.GH., TALEBI M., BAGHERIAN R., HAJI HOSSEINI R.. A SIMPLE, FAST AND COMMERCIAL METHOD FOR TRYPSIN PURIFICATION FROM BOVINE PANCREAS. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI)[Internet]. 2011;24(1 (90)):33-39. Available from: https://sid.ir/paper/200978/en

IEEE: Copy

B.GH. GOUDARZI, M. TALEBI, R. BAGHERIAN, and R. HAJI HOSSEINI, “A SIMPLE, FAST AND COMMERCIAL METHOD FOR TRYPSIN PURIFICATION FROM BOVINE PANCREAS,” VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), vol. 24, no. 1 (90), pp. 33–39, 2011, [Online]. Available: https://sid.ir/paper/200978/en

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