THE STUDY OF DISTRIBUTION AND ACTIVITY OF PROTEOLYTIC BACTERIA IN THE SURFACE AND SUB SURFACE WATER LAYERS IN SOUTH OF CASPIAN SEA

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TABATABAI NASAB M.

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Journal: PEYKE NOOR JOURNAL SCIENCE Year:2007 | Volume:1 | Issue:3 Page(s): 111-118

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Title

THE STUDY OF DISTRIBUTION AND ACTIVITY OF PROTEOLYTIC BACTERIA IN THE SURFACE AND SUB SURFACE WATER LAYERS IN SOUTH OF CASPIAN SEA

Author(s)

TABATABAI NASAB M. | Issue Writer Certificate

Keywords

PROTEOLYTIC BACTERIAQ3

PROTEASEQ3

CASPIAN SEAQ3

Abstract

Protease enzymes which are very important in different industries, change proteins to simple ingredients and amino acids, enzymes in wide varieties of industries such as pharmacy, food, medical, etc.Many kinds of bacteria such as Bacillus spp and Aearomonsa spp have the ability to produce these enzymes.In this research, samples have been taken from southern area of CASPIAN SEA in order to detach the PROTEOLYTIC BACTERIA and culture was made from these samples on the milk agar. Then samples were incubated about 48 hours, at 30oc. Then the clones have been considered numerically, morphologically & physiologically.In the next stage in order to detach of bacterial PROTEASE, the detached PROTEASE positive bacteria in skim milk 1% medium were cultivated and kept at 30oc for 13 days with shaking at 150 rpm. After that, samples were put in centrifuge at 1000 rpm for 10 minutes, Azokasein 2%, was added as substrate, sodium buffer phosphate 10% Tricloro acetic acid 1M and absorption was read in 595 nm and protein concentration was calculated in Brad ford method. Therefore purified sample was in SDS- PAGE method and in the next stage in order to gain optimized PH, salt density variation, and temperature effects on enzyme production were deliberated. One of those bacteria had a higher ability to produce PROTEASE under that condition. The Further results of genus and species showed that the final bacteria is Flavimonas oryzihabitans. The best result to produce the enzyme, demonstrated at 30oC pH 7.2 and salt concentration at 0.2 – 2.5 M.

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APA: Copy

TABATABAI NASAB, M.. (2007). THE STUDY OF DISTRIBUTION AND ACTIVITY OF PROTEOLYTIC BACTERIA IN THE SURFACE AND SUB SURFACE WATER LAYERS IN SOUTH OF CASPIAN SEA. PEYKE NOOR JOURNAL SCIENCE, 1(3), 111-118. SID. https://sid.ir/paper/203486/en

Vancouver: Copy

TABATABAI NASAB M.. THE STUDY OF DISTRIBUTION AND ACTIVITY OF PROTEOLYTIC BACTERIA IN THE SURFACE AND SUB SURFACE WATER LAYERS IN SOUTH OF CASPIAN SEA. PEYKE NOOR JOURNAL SCIENCE[Internet]. 2007;1(3):111-118. Available from: https://sid.ir/paper/203486/en

IEEE: Copy

M. TABATABAI NASAB, “THE STUDY OF DISTRIBUTION AND ACTIVITY OF PROTEOLYTIC BACTERIA IN THE SURFACE AND SUB SURFACE WATER LAYERS IN SOUTH OF CASPIAN SEA,” PEYKE NOOR JOURNAL SCIENCE, vol. 1, no. 3, pp. 111–118, 2007, [Online]. Available: https://sid.ir/paper/203486/en

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