COMPARISON AND OPTIMIZATION OF METHODS FOR ISOLATION OF NON-TUBERCULOUS MYCOBACTERIA FROM SURFACE WATER

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

FALSAFI SARVENAZ; ZAKERBOSTANABAD SAIED; FEIZABADI MOHAMMAD MEHDI; KHAVARI NEJAD RAMEZAN ALI

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2014 | Volume:5 | Issue:15 Page(s): 115-121

Download Full-Text

Persian Verion

View:

944

Download:

Cites:

Information Journal Paper

Title

COMPARISON AND OPTIMIZATION OF METHODS FOR ISOLATION OF NON-TUBERCULOUS MYCOBACTERIA FROM SURFACE WATER

Author(s)

Keywords

AQUATIC ECOSYSTEMSQ2

DECONTAMINATION METHODSQ2

NONTUBERCULOUS MYCOBACTERIA (NTM)Q1

Abstract

Aim and Background: Environment is the likely source of most Non-Tuberculous mycobacteria (NTM) involved in human infections, especially pulmonary, skin, and soft tissue infections. In order to measure the prevalence of NTM in different AQUATIC ECOSYSTEMS, we tried to standardize the isolation methods used for surface water testing since many procedures have been described previously. Cultivation of mycobacteria requires long-term incubation in rich media and inactivation of rapidly growing microorganisms whose growth impedes observation of mycobacterial colonies.Materials and Methods: 38 samples were collected from surface waters in Tehran. Water sampling was carried out in a volume of 200-100 ml. water sample was transferred immediately to the laboratory and examined. Three methods (Cetylpyridinium chlori, Petroff, Tacquet-Tison) for the isolation of mycobacteria were compared by applying them in parallel to 38 samples of surface water. Each method was defined by a particular combination of decontamination method. The efficacy of each method was determined by calculating the positivity, negativity, and contamination rates, the mean numbers of mycobacterial colonies grown and number of different mycobacterial strains isolated. The last value was determined by subjecting the isolates to PCR.Results: Decontamination with CPC appeared to be the best decontamination method, on the one hand, it significantly decreased the level of non-target microorganisms and, on the other hand, it was significantly less lethal for the NTM strains studied.Conclusion: Our goal was to measure the effects of various methods known to inhibit the growth of non-target microorganisms, while we also took into consideration the inhibitory effects of these methods on the growth of NTM. We propose that CPC procedure could be used for detection of NTM in aquatic samples.

Cites

No record.

References

No record.

Cite

APA: Copy

FALSAFI, SARVENAZ, ZAKERBOSTANABAD, SAIED, FEIZABADI, MOHAMMAD MEHDI, & KHAVARI NEJAD, RAMEZAN ALI. (2014). COMPARISON AND OPTIMIZATION OF METHODS FOR ISOLATION OF NON-TUBERCULOUS MYCOBACTERIA FROM SURFACE WATER. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 5(15), 115-121. SID. https://sid.ir/paper/204380/en

Vancouver: Copy

FALSAFI SARVENAZ, ZAKERBOSTANABAD SAIED, FEIZABADI MOHAMMAD MEHDI, KHAVARI NEJAD RAMEZAN ALI. COMPARISON AND OPTIMIZATION OF METHODS FOR ISOLATION OF NON-TUBERCULOUS MYCOBACTERIA FROM SURFACE WATER. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2014;5(15):115-121. Available from: https://sid.ir/paper/204380/en

IEEE: Copy

SARVENAZ FALSAFI, SAIED ZAKERBOSTANABAD, MOHAMMAD MEHDI FEIZABADI, and RAMEZAN ALI KHAVARI NEJAD, “COMPARISON AND OPTIMIZATION OF METHODS FOR ISOLATION OF NON-TUBERCULOUS MYCOBACTERIA FROM SURFACE WATER,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 5, no. 15, pp. 115–121, 2014, [Online]. Available: https://sid.ir/paper/204380/en

Related Journal Papers