Computational study of Housekeeping Genes in Differentiation of Non-Tuberculosis Mycobacteria

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KEIKHA MASOUD; SHAHRAKI ZAHEDANI SHAHRAM; Rahdar Hossein Ali

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Journal: Journal of Sabzevar University of Medical Sciences Year:2019 | Volume:25 | Issue:5 Page(s): 679-686

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Information Journal Paper

Title

Computational study of Housekeeping Genes in Differentiation of Non-Tuberculosis Mycobacteria

Author(s)

KEIKHA MASOUD | SHAHRAKI ZAHEDANI SHAHRAM | Rahdar Hossein Ali | Issue Writer Certificate

Keywords

hsp65Q1

non-tuberculosis mycobacteriaQ1

rpoBQ2

16S rRNAQ2

Abstract

Background and Objectives: non-tuberculosis mycobacteria is acid-fast bacteria that lives in environmental sources such as water, soil, dust, milk and decaying vegetables. Based on Runyon’ s classification, these bacteria classified in tow group of slow and rapid growing mycobacteria; Both of them isolated from clinical specimens. The purpose of this study is evaluation of three housekeeping genes in identification and differentiation of non-tuberculosis mycobacteria. Materials & Methods: In our study, we obtained 16S rRNA, rpoB and hsp65 sequences of 22 slow and rapid growing mycobacteria from Genebank (www. ncbi. nlm. nih. gov). Then, these sequences aligned and transferred to MEGA 5. 0 program. Finally, phylogenetic relationships were determined by constructing 16S rRNA, rpoB and hsp65 genes tree using the neighbor-joining method with Kimura 2-Parameter model. Results and Conclustion Phylogenetic analysis based on 16S rRNA, rpoB and hsp65 gene sequences indicated that except of rpoB gene. Other genes cannot identificate and separate of some species. Also we found that for identification both of rapid growth mycobacteria (RGM) and slow growth mycobacteria (SGM) rpoB gene is the best option. Due to findings of this study, it seems that for appropriate and accurate identification of non-tuberculosis mycobacteria, we must studied rpoB and hsp65 genes simultaneously.

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APA: Copy

KEIKHA, MASOUD, SHAHRAKI ZAHEDANI, SHAHRAM, & Rahdar, Hossein Ali. (2019). Computational study of Housekeeping Genes in Differentiation of Non-Tuberculosis Mycobacteria. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, 25(5 ), 679-686. SID. https://sid.ir/paper/82133/en

Vancouver: Copy

KEIKHA MASOUD, SHAHRAKI ZAHEDANI SHAHRAM, Rahdar Hossein Ali. Computational study of Housekeeping Genes in Differentiation of Non-Tuberculosis Mycobacteria. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;25(5 ):679-686. Available from: https://sid.ir/paper/82133/en

IEEE: Copy

MASOUD KEIKHA, SHAHRAM SHAHRAKI ZAHEDANI, and Hossein Ali Rahdar, “Computational study of Housekeeping Genes in Differentiation of Non-Tuberculosis Mycobacteria,” JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, vol. 25, no. 5 , pp. 679–686, 2019, [Online]. Available: https://sid.ir/paper/82133/en

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