Identification of Mycoplasma gallisepticum in rural domestic poultry of Urmia city in the year 1395

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HOSSEINI NASRIN; DILMAGHANI MAHDI

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2018 | Volume:8 | Issue:32 Page(s): 43-52

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Title

Identification of Mycoplasma gallisepticum in rural domestic poultry of Urmia city in the year 1395

Author(s)

HOSSEINI NASRIN | DILMAGHANI MAHDI | Issue Writer Certificate

Keywords

Mycoplasma gallisepticumQ1

Rapid Serum AgglutinationQ1

Rural domestic poultryQ1

PCRQ2

16S rRNA geneQ1

Abstract

Aim and BacKground: Mycoplasma gallisepticum is the most economically significant mycoplasma pathogen of poultry, and has a world-wide distribution. Infection with M. gallisepticum has a wide variety of clinical manifestations, but even in the absence of overt clinical signs, the economic impact may be significant. Materials and methods: Blood and swab samples were collected from 145 birds in sterile condition and transferred to lab with ice. Rapid Agglutination test was performed on blood samples and PCR assay targeting 16S rRNA was done on swab samples following DNA extraction. Results: Based on Rapid Serum Agglutination Test on 145 samples, 24/8% of Rural domestic poultry were positive and PCR using 16S rRNA gene primers denoted that 63/4%of samples are positive. Conclusion: Reliable methods for the discrimination of MG strains play an essential role in understanding the epidemiology and spread of the disease because they generate the information required for recognising and following new outbreaks. Genotyping methods also have a high degree of reproducibility, which is particularly important for the construction of reliable databases. Furthermore, genotyping methods are easy to interpret and can be performed rapidly.

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APA: Copy

HOSSEINI, NASRIN, & DILMAGHANI, MAHDI. (2018). Identification of Mycoplasma gallisepticum in rural domestic poultry of Urmia city in the year 1395. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 8(32 ), 43-52. SID. https://sid.ir/paper/204396/en

Vancouver: Copy

HOSSEINI NASRIN, DILMAGHANI MAHDI. Identification of Mycoplasma gallisepticum in rural domestic poultry of Urmia city in the year 1395. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2018;8(32 ):43-52. Available from: https://sid.ir/paper/204396/en

IEEE: Copy

NASRIN HOSSEINI, and MAHDI DILMAGHANI, “Identification of Mycoplasma gallisepticum in rural domestic poultry of Urmia city in the year 1395,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 8, no. 32 , pp. 43–52, 2018, [Online]. Available: https://sid.ir/paper/204396/en

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