THE EFFECT OF GLUCOSE AND CITRIC ACID ON BOVINE SPERM MOTILITY PATTERNS

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ASADI ELHAM

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2015 | Volume:5 | Issue:20 Page(s): 105-111

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Title

THE EFFECT OF GLUCOSE AND CITRIC ACID ON BOVINE SPERM MOTILITY PATTERNS

Author(s)

ASADI ELHAM | Issue Writer Certificate

Keywords

EPIDIDYMAL SPERMQ2

GLUCOSEQ2

CITRIC ACIDQ1

VIABILITYQ2

VELOCITYQ2

Abstract

Aim and background Sperm motility is a critical factor for fertilization. GLUCOSE and CITRIC ACID can be metabolized by these cells. We study the role of these two on Bovine sperm motility in BO medium.Materials and methods Testicles were removed from tunica albuginea. Epididymes were incised and 4-6 *106 sperm cells/ml were transferred into BO medium containing different concentrations of GLUCOSE (G1 & G2) with or without CITRIC ACID, and incubated in a %5 Co2 incubator in humid air at 38 C for up to 8 hours.Sperm cells were observed under CASA system (computer assisted sperm analyzer) and data for the Class A, Class A+B, Live ratio, Straight Line VELOCITY (VSL), Curvilinear VELOCITY (VCL), Average Path VELOCITY (VAP), Beat Cross Frequency (BCF) were recorded.Results The results showed that Class A was significantly higher at G1 & CITRIC ACID at 6 hours post incubation (p<0.05). Class A+B was significantly higher at G1 & CITRIC ACID at 4 & 6 hours post incubation (p<0.05). Live ratio was significantly higher at G1 & CITRIC ACID too. Such effects didn’t observe at G2 & CITRIC ACID medium.Conclusion The results showed that CITRIC ACID has an increasing effect on these parameters. This effect can be affected by concentrations of GLUCOSE. Our results didn’t show any significant difference in comparing motility patterns atG1 and G2 concentrations of GLUCOSE with or without CITRIC ACID. This part of the results suggests that CITRIC ACID has no any effect on these parameters.

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APA: Copy

ASADI, ELHAM. (2015). THE EFFECT OF GLUCOSE AND CITRIC ACID ON BOVINE SPERM MOTILITY PATTERNS. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 5(20), 105-111. SID. https://sid.ir/paper/204455/en

Vancouver: Copy

ASADI ELHAM. THE EFFECT OF GLUCOSE AND CITRIC ACID ON BOVINE SPERM MOTILITY PATTERNS. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2015;5(20):105-111. Available from: https://sid.ir/paper/204455/en

IEEE: Copy

ELHAM ASADI, “THE EFFECT OF GLUCOSE AND CITRIC ACID ON BOVINE SPERM MOTILITY PATTERNS,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 5, no. 20, pp. 105–111, 2015, [Online]. Available: https://sid.ir/paper/204455/en

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