PARTIAL PURIFICATION OF PEROXIDASE IN CORMS OF SAFFRON (CRUCUS SATIVUS L.) DURING DORMANCY AND WAKING

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RAHMANI A.; SEIGHALI N.; EBRAHIMZADEH H.; ZAREI JALIANI H.

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2012 | Volume:2 | Issue:8 Page(s): 89-95

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Title

PARTIAL PURIFICATION OF PEROXIDASE IN CORMS OF SAFFRON (CRUCUS SATIVUS L.) DURING DORMANCY AND WAKING

Author(s)

RAHMANI A. | SEIGHALI N. | EBRAHIMZADEH H. | ZAREI JALIANI H. | Issue Writer Certificate

Keywords

CROCUS SATIVUS LQ2

PEROXIDASEQ2

DORMANCYQ2

WAKINGQ2

Abstract

Aim and Background: PEROXIDASEs are one of the most important enzymes participating in many physiological processes in plants. Saffron, (CROCUS SATIVUS L), is a valuable plant which has been grown since ancient times to date. But there is a lack of sufficient biochemical and physiological information about the stages of the saffron growth, development and the important enzymes involved. PEROXIDASEs in CROCUS SATIVUS L. can be a marker of distinct physiological ordevelopmental processes, as shown before by laboratory studies.Materials and Methods: In this study, the Crucus Sativus L. corms in two different months –July and November as dormant and WAKING stages of saffron growth, sampled and Corms were used for the enzyme extraction. It was partially purified with ion-exchange chromatography.Results: The results showed that amount of total protein in WAKING corms is about two times in comparison with the dormant corms. The high activity of the plant in WAKING state corresponds with more protein content.Conclusion: This difference might be partially due to the different patterns of the enzymeglycosylation and surface electrostatic charges.

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APA: Copy

RAHMANI, A., SEIGHALI, N., EBRAHIMZADEH, H., & ZAREI JALIANI, H.. (2012). PARTIAL PURIFICATION OF PEROXIDASE IN CORMS OF SAFFRON (CRUCUS SATIVUS L.) DURING DORMANCY AND WAKING. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 2(8), 89-95. SID. https://sid.ir/paper/204506/en

Vancouver: Copy

RAHMANI A., SEIGHALI N., EBRAHIMZADEH H., ZAREI JALIANI H.. PARTIAL PURIFICATION OF PEROXIDASE IN CORMS OF SAFFRON (CRUCUS SATIVUS L.) DURING DORMANCY AND WAKING. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2012;2(8):89-95. Available from: https://sid.ir/paper/204506/en

IEEE: Copy

A. RAHMANI, N. SEIGHALI, H. EBRAHIMZADEH, and H. ZAREI JALIANI, “PARTIAL PURIFICATION OF PEROXIDASE IN CORMS OF SAFFRON (CRUCUS SATIVUS L.) DURING DORMANCY AND WAKING,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 2, no. 8, pp. 89–95, 2012, [Online]. Available: https://sid.ir/paper/204506/en

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