PURIFICATION OF PEROXIDASE FROM RAPHANUS SATIVUS VAR NIGGERA

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MOSTAFAIE A.; RAHI H.; NASERI M.

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Paper Information

Journal: Journal of Kermanshah University of Medical Sciences Year:2000 | Volume:4 | Issue:1 Page(s): 8-16

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Information Journal Paper

Title

PURIFICATION OF PEROXIDASE FROM RAPHANUS SATIVUS VAR NIGGERA

Author(s)

MOSTAFAIE A. | RAHI H. | NASERI M. | Issue Writer Certificate

Keywords

PEROXIDAZEQ4

PURIFICATIONQ3

RAPHANUS SATIVUS VAR NIGGERAQ4

Abstract

Peroxidase ((PRO) (EC.1.11.1.7)) has applications in many diagnostic procedures such as ELISA and immunoperoxidase. Concerning HRP related products; we are dependent to other countries. Therefore the PURIFICATION of PRO can be an economically important object. RAPHANUS SATIVUS VAR NIGGERA was tested to be a suitable source for PRO PURIFICATION. The steps of PURIFICATION were included: extraction by phosphate buffer (0.05 M, pH 7), ethanol and ammonium sulfate precipitation and ion-exchange chromatography on DEAE-Cellulose. Based on the affinity to DEAE-Cellulose column a major alkalin form and one minor acidic form of PRO were detected. The fold of PURIFICATION was 102 and the recovery was 67%. The molecular weight and Rz for the alkalin isoenzyme were calculated to be 40000 daltons and 1.95, respectively. The extent of enzyme purity was verified by SDS-PAGE. Regarding the economical point of view, mass production of this enzyme as internal product is suggested.

Cites

PURIFICATION AND PARTIAL CHARACTERIZATION OF BARLEY OXALATE OXIDASE

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APA: Copy

MOSTAFAIE, A., RAHI, H., & NASERI, M.. (2000). PURIFICATION OF PEROXIDASE FROM RAPHANUS SATIVUS VAR NIGGERA. JOURNAL OF KERMANSHAH UNIVERSITY OF MEDICAL SCIENCES (BEHBOOD), 4(1), 8-16. SID. https://sid.ir/paper/20755/en

Vancouver: Copy

MOSTAFAIE A., RAHI H., NASERI M.. PURIFICATION OF PEROXIDASE FROM RAPHANUS SATIVUS VAR NIGGERA. JOURNAL OF KERMANSHAH UNIVERSITY OF MEDICAL SCIENCES (BEHBOOD)[Internet]. 2000;4(1):8-16. Available from: https://sid.ir/paper/20755/en

IEEE: Copy

A. MOSTAFAIE, H. RAHI, and M. NASERI, “PURIFICATION OF PEROXIDASE FROM RAPHANUS SATIVUS VAR NIGGERA,” JOURNAL OF KERMANSHAH UNIVERSITY OF MEDICAL SCIENCES (BEHBOOD), vol. 4, no. 1, pp. 8–16, 2000, [Online]. Available: https://sid.ir/paper/20755/en

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