EXPRESSION OF RECOMBINANT HUMAN SERUM ALBUMIN IN E. COLI (BL21)

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GHAFARI F.; KHEIRANDISH F.; KAZEMI B.; GHAFARI M.D.; BANDEHPOUR M.

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2013 | Volume:3 | Issue:9 Page(s): 61-66

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Title

EXPRESSION OF RECOMBINANT HUMAN SERUM ALBUMIN IN E. COLI (BL21)

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Keywords

HUMAN SERUM ALBUMINQ2

BL21 (DE3)Q2

PET22BQ2

Abstract

Aim and Background: HUMAN SERUM ALBUMIN (HSA) is the most important components of the circulatory system. HSA can transport many metabolites and hormones in the body. It is effective for blood volume stabilization and the bum treatment. The preparation of HSA from human plasma is costly and the risk of contamination with various pathogens is high. Therefore, biotechnology could promise a new way to solve the problems.Materials and Methods: HSA gene was synthesized based on the sequence of the gene in NeBI website. HSA was cloned into PET22B expression vector and then the recombinant plasmid was transformed into BL2l (DE3) competent cell. The colony (containing recombinant expression vector) was cultured overnight. The culture was induced with IPTG 1.5 mM in the logarithmic phase (OD600=0.6). Sampling was carried out in different times and it was analyzed on %10 (w/v) SDS- PAGE. The expression of recombinant HUMAN SERUM ALBUMIN (rHSA) was confirmed by western blot. Finally, rHSA was purified by affinity chromatography.Results: In this study, HUMAN SERUM ALBUMIN was produced in the bacterial periplasmic environment. The highest expression level of rHSA was observed 24 h after the bacterial induction. Conclusion: In this study, rHSA was produced in E. coli host.

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APA: Copy

GHAFARI, F., KHEIRANDISH, F., KAZEMI, B., GHAFARI, M.D., & BANDEHPOUR, M.. (2013). EXPRESSION OF RECOMBINANT HUMAN SERUM ALBUMIN IN E. COLI (BL21). NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 3(9), 61-66. SID. https://sid.ir/paper/204528/en

Vancouver: Copy

GHAFARI F., KHEIRANDISH F., KAZEMI B., GHAFARI M.D., BANDEHPOUR M.. EXPRESSION OF RECOMBINANT HUMAN SERUM ALBUMIN IN E. COLI (BL21). NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2013;3(9):61-66. Available from: https://sid.ir/paper/204528/en

IEEE: Copy

F. GHAFARI, F. KHEIRANDISH, B. KAZEMI, M.D. GHAFARI, and M. BANDEHPOUR, “EXPRESSION OF RECOMBINANT HUMAN SERUM ALBUMIN IN E. COLI (BL21),” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 3, no. 9, pp. 61–66, 2013, [Online]. Available: https://sid.ir/paper/204528/en

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