THE DEVELOPMENT OF A PCR METHOD FOR MYCOPLASMA TESTING IN CELL CULTURE

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GHIASI MAHDIEH SADAT; SHAH HOSSEINI MOHAMMAD HASAN; MOHAJERANI HAMID REZA

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2014 | Volume:4 | Issue:14 Page(s): 41-46

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Title

THE DEVELOPMENT OF A PCR METHOD FOR MYCOPLASMA TESTING IN CELL CULTURE

Author(s)

GHIASI MAHDIEH SADAT | SHAH HOSSEINI MOHAMMAD HASAN | MOHAJERANI HAMID REZA | Issue Writer Certificate

Keywords

MYCOPLASMAQ2

PCRQ2

CONTAMINATIONQ2

MOLECULAR DETECTIONQ1

CELL CULTUREQ2

Abstract

Aim and Background: CONTAMINATION of cell lines and biological products is one of themajor problemsof CELL CULTURE techniques. Rapid detection of MYCOPLASMA CONTAMINATION i ncell culture is an important part of controlled laboratory, and shouldthere bea method in laboratory CELL CULTUREs. Infected cells, leading to unreliable results, so the need for areliable method for laboratories is essential. Polymerase chain reaction, a technique ofrapid, sensitive and spec if icdetection of bacteriais considered. The aim ofthisstudy was to evaluate the efficacy of PCR in the detection of pollutants in CELL CULTUREsand other biological products Materials and Methods: Inthisstudy, PCR techniques using primers MGSO and GPO-1 and target gene 16Sr RNA was optimized. Also used PCR method was evaluated in terms of sensitivity and specificity. Finally, a simple DNA extractionand PCRof 72 cell lines tested.Result: A 715 bpproduct was amplified by the primers and was confirmed by sequencing. The Reviews feature, withnone ofthe tested DNA was amplified products. This method has a sensitivity limit of 10 copies of the target DNA. No cross-reactivity with genomic DNA of other microorganisms was observed.Conclusion: The results of this study indicate that the molecular methods for detection of Myoplasma CONTAMINATION in CELL CULTUREs or Biologic Products of allergy and high specificity is.

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APA: Copy

GHIASI, MAHDIEH SADAT, SHAH HOSSEINI, MOHAMMAD HASAN, & MOHAJERANI, HAMID REZA. (2014). THE DEVELOPMENT OF A PCR METHOD FOR MYCOPLASMA TESTING IN CELL CULTURE. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 4(14), 41-46. SID. https://sid.ir/paper/204540/en

Vancouver: Copy

GHIASI MAHDIEH SADAT, SHAH HOSSEINI MOHAMMAD HASAN, MOHAJERANI HAMID REZA. THE DEVELOPMENT OF A PCR METHOD FOR MYCOPLASMA TESTING IN CELL CULTURE. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2014;4(14):41-46. Available from: https://sid.ir/paper/204540/en

IEEE: Copy

MAHDIEH SADAT GHIASI, MOHAMMAD HASAN SHAH HOSSEINI, and HAMID REZA MOHAJERANI, “THE DEVELOPMENT OF A PCR METHOD FOR MYCOPLASMA TESTING IN CELL CULTURE,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 4, no. 14, pp. 41–46, 2014, [Online]. Available: https://sid.ir/paper/204540/en

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