مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

1,034
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

0
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

THE COMPARISON OF MICROBIAL CULTURE AND PCR METHODS IN DETECTION OF CELL LINE TO MYCOPLASMA

Pages

  1676-1683

Abstract

 Background: MYCOPLASMA is a major contaminant agent of CELL LINES and is cosiderd as a serious problem of economic and biological importance in basic research, diagnosis, and biotechnology products. Detection of MYCOPLASMA infection in cell cultures started on MICROBIOLOGICAL CULTURE; later, other methods like DAPI staining and serological tests such as Indirect Immunofluorescnse, ELISA, DNA probe, PCR, PCR-ELISA, and Real-Time PCR developed for detection of MYCOPLASMA. In this study, we detect the contamination of varity of MYCOPLASMA species in CELL LINES in national cell bank of Iran.Methods: In this study, a sensitive, specific, and rapid method was used for detection of varity of MYCOPLASMA species in CELL LINES. This method was based on a PCR reaction using genus specific primers for 11 MYCOPLASMA species.Results: MYCOPLASMA contamination using this assay was examind for 183 different CELL LINES deposited in national cell bank of Iran. PCR showed that 48.6% of CELL LINES were contaminated with MYCOPLASMA while 27.3% of them were found to be infected by microbial culture. In comparison to MICROBIOLOGICAL CULTURE, PCR method was shown to be 100% sensitive and 70.7% specific.Conclusion: Our results using species specific primers reveald that the most important contaminating MYCOPLASMA species in CELL LINES were MYCOPLASMA fermentans, MYCOPLASMA arginini, MYCOPLASMA hyorhinis, and MYCOPLASMA orale. We were also able to identify a number of CELL LINES which were contaminated whit more than one species of MYCOPLASMA.

Cites

  • No record.
  • References

  • No record.
  • Cite

    APA: Copy

    ARABESTANI, MOHAMMAD REZA, FAZELI, HOSSAIN, JEDI TEHRANI, MAHMMUD, & SHOKRI, FAZEL. (2011). THE COMPARISON OF MICROBIAL CULTURE AND PCR METHODS IN DETECTION OF CELL LINE TO MYCOPLASMA. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 28(121), 1676-1683. SID. https://sid.ir/paper/50790/en

    Vancouver: Copy

    ARABESTANI MOHAMMAD REZA, FAZELI HOSSAIN, JEDI TEHRANI MAHMMUD, SHOKRI FAZEL. THE COMPARISON OF MICROBIAL CULTURE AND PCR METHODS IN DETECTION OF CELL LINE TO MYCOPLASMA. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2011;28(121):1676-1683. Available from: https://sid.ir/paper/50790/en

    IEEE: Copy

    MOHAMMAD REZA ARABESTANI, HOSSAIN FAZELI, MAHMMUD JEDI TEHRANI, and FAZEL SHOKRI, “THE COMPARISON OF MICROBIAL CULTURE AND PCR METHODS IN DETECTION OF CELL LINE TO MYCOPLASMA,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 28, no. 121, pp. 1676–1683, 2011, [Online]. Available: https://sid.ir/paper/50790/en

    Related Journal Papers

    Related Seminar Papers

  • No record.
  • Related Plans

  • No record.
  • Recommended Workshops






    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    File Not Exists.
    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button