Molecular identification of coding genes of beta-lactamase (CTX-M) in Klebsiella pneumoniae isolates from patients reffer to hospitals of Tehran city

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Shamlou Niloufar; ZAKER BOSTANABAD SAEED; MIRNEJAD REZA

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2018 | Volume:8 | Issue:30 Page(s): 25-34

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Information Journal Paper

Title

Molecular identification of coding genes of beta-lactamase (CTX-M) in Klebsiella pneumoniae isolates from patients reffer to hospitals of Tehran city

Author(s)

Shamlou Niloufar | ZAKER BOSTANABAD SAEED | MIRNEJAD REZA | Issue Writer Certificate

Keywords

Klebsiella pneumoniaeQ2

ExtendedQ1

spectrum betaQ1

lactamase bla CTXQ1

M geneQ1

Abstract

Aim and Background: Extended-spectrum beta-lactamase of CTX-M type is considered as an Important mechanism resistant to beta-lactamases in gram-negative pathogen and is widely increasing. Klebsiella pneumoniae species are able to produce Extended-spectrum beta-lactamase (ESBLs). The aim of this study was to detect the prevalence of CTX-M gene in ESBLs produsing Klebsiella pneumoniae isolated form patients in different region of TEHRAN city from December2016 to july 2017 by using PCR method. Material and methods: In this analytical-descriptive study antibacterial susceptibility patterns of 176 Klebsiella pneumoniae tested to Cefepime, Ampicillin, Gentamicin, Amikacin, Cefalotine, Ceftazidime, Piperacillin, Ciprofloxacin, Imipenem, Cefotaxime, Nitrofurantoin, Trimethoprim using disk diffusion method. In addition, confirmatory test for detecting ESBLs phenotypes was performed using Cefotaxim-Clavulanic acid combination disk. The presence of CTX-M gene was assessed by using PCR. Results: Confirmatory phenotypic test showed 56. 81% of the isolates were ESBL positive. The prevalence of CTX-M gene in isolated Klebsiella pneumoniae was 28%. In this study, the highest resistance was observed for ampicillin antibiotics (24. 88%) and the least resistance to antibiotic imipenem (6. 06). Conclusion: High frequency of CTX-M gene in ESBL produsing isolates indicates that this enzyms plays an important role in resistance to beta lactam containing anti biotics. Therefore, proper infection control tools and appropriate therapeutic approaches in different parts of the hospitals are necessary to prevent their release.

Cites

Molecular Diagnostic of Drug Resistant Klebsiella Pneumoniae Isolates Containing SHV, PER and VEB Genes in Samples Collected from Patients in Tehran

References

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APA: Copy

Shamlou, Niloufar, ZAKER BOSTANABAD, SAEED, & MIRNEJAD, REZA. (2018). Molecular identification of coding genes of beta-lactamase (CTX-M) in Klebsiella pneumoniae isolates from patients reffer to hospitals of Tehran city. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 8(30 ), 25-34. SID. https://sid.ir/paper/204550/en

Vancouver: Copy

Shamlou Niloufar, ZAKER BOSTANABAD SAEED, MIRNEJAD REZA. Molecular identification of coding genes of beta-lactamase (CTX-M) in Klebsiella pneumoniae isolates from patients reffer to hospitals of Tehran city. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2018;8(30 ):25-34. Available from: https://sid.ir/paper/204550/en

IEEE: Copy

Niloufar Shamlou, SAEED ZAKER BOSTANABAD, and REZA MIRNEJAD, “Molecular identification of coding genes of beta-lactamase (CTX-M) in Klebsiella pneumoniae isolates from patients reffer to hospitals of Tehran city,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 8, no. 30 , pp. 25–34, 2018, [Online]. Available: https://sid.ir/paper/204550/en

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