2018131Isolation and molecular identification of resistant bacteria Pb from soils oil contaminated in area C-branch Masjed Soleiman city in Khuzestan

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Sajadian Maedeh; DOUDI MONIR; Shirmardi Abbas

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Paper Information

Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2018 | Volume:8 | Issue:30 Page(s): 71-78

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Title

2018131Isolation and molecular identification of resistant bacteria Pb from soils oil contaminated in area C-branch Masjed Soleiman city in Khuzestan

Author(s)

Sajadian Maedeh | DOUDI MONIR | Shirmardi Abbas | Issue Writer Certificate

Keywords

Heavy metal resistant bacteriaQ1

PbQ2

Oil contaminated soilsQ1

Molecular identificationQ2

Minimum inhibitory concentration (MIC)Q1

Abstract

Aim and Background: Due to the high prevalence of diseases caused by the presence of lead in soil contaminated with petroleum industrial centers, then transfer the metal to plants and animals and eventually humans and high risks for human health and other living things. It seems that identify and isolate microorganisms with high strength steel and the biological removal can be an important step in the evolution of contaminated soil cleanup systems considered industrial centers. Material and methods: The was sampling of three areas of Oil contaminated soils C-branch Masjed Soleiman. To isolate resistant bacteria lead Ι Ι PHG agar dilution method and the culture medium containing concentrations of 0. 5, 1, 2, 2. 5, 3, 3. 5 and 4 mMol per liter of lead were used. To identify of resistant bacteria lead, first was used of biochemical tests and then molecular methods PCR-colony. Results: In this study, 5 resistant bacteria lead from Oil contaminated soils were isolated and identified that among of them Proteus mirabilis strain AOUC-001 bacteria and showed maximum resistance to lead (MIC=4mM). Conclusion: According to the bacteria examined in this study could lead bioabsorption in-vitro and growth rather appropriate in the presence of the metal showed, so it seems a good candida for bioremedation of lead from Oil contaminated soils in the future. Provided that the contaminated soils to isolate the bacteria to be filtered out treatment.

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References

THE STUDY OF NICKEL RESISTANT BACTERIA (NIRB) ISOLATED FROM WASTEWATERS POLLUTED WITH DIFFERENT INDUSTRIAL SOURCES

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APA: Copy

Sajadian, Maedeh, DOUDI, MONIR, & Shirmardi, Abbas. (2018). 2018131Isolation and molecular identification of resistant bacteria Pb from soils oil contaminated in area C-branch Masjed Soleiman city in Khuzestan. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 8(30 ), 71-78. SID. https://sid.ir/paper/204561/en

Vancouver: Copy

Sajadian Maedeh, DOUDI MONIR, Shirmardi Abbas. 2018131Isolation and molecular identification of resistant bacteria Pb from soils oil contaminated in area C-branch Masjed Soleiman city in Khuzestan. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2018;8(30 ):71-78. Available from: https://sid.ir/paper/204561/en

IEEE: Copy

Maedeh Sajadian, MONIR DOUDI, and Abbas Shirmardi, “2018131Isolation and molecular identification of resistant bacteria Pb from soils oil contaminated in area C-branch Masjed Soleiman city in Khuzestan,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 8, no. 30 , pp. 71–78, 2018, [Online]. Available: https://sid.ir/paper/204561/en

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