ISOLATION AND MOLECULAR IDENTIFICATION OF NICKEL AND CADMIUM RESISTANT BACTERIA FROM GUILAN INDUSTRIAL WASTEWATER

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SIMIARY F.; NAZEMI A.; NASROLAHI A.

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Journal: Biotechnological Journal of Environmental Microorganisms Year:2012 | Volume: | Issue: Page(s): 43-49

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Title

ISOLATION AND MOLECULAR IDENTIFICATION OF NICKEL AND CADMIUM RESISTANT BACTERIA FROM GUILAN INDUSTRIAL WASTEWATER

Author(s)

SIMIARY F. | NAZEMI A. | NASROLAHI A. | Issue Writer Certificate

Keywords

MOLECULAR IDENTIFICATIONQ2

NICKEL AND CADMIUM RESISTANT BACTERIAQ2

INDUSTRIAL WASTEWATERQ2

Abstract

Nickel and Cadmium are among the heavy and toxic metals whose industrial usage lead to environment pollution extensively. One of the best procedures in removing of the toxic minerals in nature is using metal resistant bacteria. Therefore, determining the bacteria resistant to the toxic minerals is the first step in usage of them in bioremediation. In result INDUSTRIAL WASTEWATER bioremediation is highly regarded as an economical and environmentally friendly option. In order to isolation and identification of resistant bacteria to nickel and cadmium, sampling was performed from wastewater in eight factories in the north of Iran (Guilan). Several bacterial isolates were separated on LB agar medium containing minimal concentrations of heavy metals, nickel (0.002 gr/plate) and cadmium levels (2.5 mg/plate). The primary metal resistant isolates that showed maximum resistance, nickel (0.2 gr/plate) and cadmium (20 mg/plate), were also separated. After microscopic examination, MOLECULAR IDENTIFICATION of bacteria was performed by 16S rDNA PCR Sequencing. A total, 17 different species were identified which includes Staphylococcus epidermidis, Staphylococcus pasteuri, Planococcus spp., Brevundimonas diminuta, Bacillus licheniformis, Pseudomonas spp., Exiguobacterium spp., Micrococcus spp., Comamonas spp., Delftia spp., Chryseobacterium spp. Serratia spp., Acinetobacter spp. that showed the highest resistance to nickel (0.2 gr/plate) and cadmium (20 mg / plate).

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APA: Copy

SIMIARY, F., NAZEMI, A., & NASROLAHI, A.. (2012). ISOLATION AND MOLECULAR IDENTIFICATION OF NICKEL AND CADMIUM RESISTANT BACTERIA FROM GUILAN INDUSTRIAL WASTEWATER. BIOTECHNOLOGICAL JOURNAL OF ENVIRONMENTAL MICROORGANISMS, 6(2 (2)), 43-49. SID. https://sid.ir/paper/242216/en

Vancouver: Copy

SIMIARY F., NAZEMI A., NASROLAHI A.. ISOLATION AND MOLECULAR IDENTIFICATION OF NICKEL AND CADMIUM RESISTANT BACTERIA FROM GUILAN INDUSTRIAL WASTEWATER. BIOTECHNOLOGICAL JOURNAL OF ENVIRONMENTAL MICROORGANISMS[Internet]. 2012;6(2 (2)):43-49. Available from: https://sid.ir/paper/242216/en

IEEE: Copy

F. SIMIARY, A. NAZEMI, and A. NASROLAHI, “ISOLATION AND MOLECULAR IDENTIFICATION OF NICKEL AND CADMIUM RESISTANT BACTERIA FROM GUILAN INDUSTRIAL WASTEWATER,” BIOTECHNOLOGICAL JOURNAL OF ENVIRONMENTAL MICROORGANISMS, vol. 6, no. 2 (2), pp. 43–49, 2012, [Online]. Available: https://sid.ir/paper/242216/en

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