RELATION OFGYRA AND PARC GENES MUTATIONS WITH FLUOROQUINOLONESRESISTANCE INESCHERICHIA COLI OF URINARY TRACT INFECTIONS

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SIASI ELHAM; HOSSIENI FARZANEH; RAHIMINIA POOYEH

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2017 | Volume:7 | Issue:25 Page(s): 91-100

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Title

RELATION OFGYRA AND PARC GENES MUTATIONS WITH FLUOROQUINOLONESRESISTANCE INESCHERICHIA COLI OF URINARY TRACT INFECTIONS

Author(s)

SIASI ELHAM | HOSSIENI FARZANEH | RAHIMINIA POOYEH | Issue Writer Certificate

Keywords

E. COLIQ1

FLUOROQINOLONESQ1

GYRA AND PARC GENESQ2

URINARY TRACT INFECTION (UTIS)Q2

Abstract

Aim and Background: Uncomplicated urinary tract infections (UTIs) are among the most common infectious diseases in human. Escherichia coli is the causal pathogen in UTIs. For treatment of UTIs, fluoroquinolones are used. FLUOROQINOLONES-resistant E. COLI was showed by alterations in the genes that encode for the quinolone target subunits.Materials and Methods: one hundred isolates of the urinary samples were collected. E. COLI isolates wereidentified by biochemical tests. The minimal inhibitory concentration (MIC) of fluoroquinolones were determined for isolates. DNA was extracted and PCR amplification was carried out for thespecific regions of GYRA AND PARC GENES. Then amplified regions were sequenced.Results: rom 100 samples, 70 samples were E. COLI, 30 samples were Kellebsiella. Evaluating MICs for 70 E. COLI isolates to fluoroquinolones, it was found that 45 (64.28%) were resistant to quinolones.Sequencing ofgyrA and parC specific subunits has revealed five single mutations in the gyrA gene and two single mutations inparC gene.Conclusion: Fluroquinolones are potent antibacterial agents for treatment of UTIs. Mutations of DNA gyrase and topoisomerase IV associate with the fluoroquinolones resistance in E. COLI. Results have indicated a relationship between MICs of the quinolones for E. COLI isolates with mutations in gyrAand parC genes. This study also signifies an association between mutations in GYRA AND PARC GENES in E. COLI isolates with fluoroquinolone resistance.

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APA: Copy

SIASI, ELHAM, HOSSIENI, FARZANEH, & RAHIMINIA, POOYEH. (2017). RELATION OFGYRA AND PARC GENES MUTATIONS WITH FLUOROQUINOLONESRESISTANCE INESCHERICHIA COLI OF URINARY TRACT INFECTIONS. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 7(25), 91-100. SID. https://sid.ir/paper/204598/en

Vancouver: Copy

SIASI ELHAM, HOSSIENI FARZANEH, RAHIMINIA POOYEH. RELATION OFGYRA AND PARC GENES MUTATIONS WITH FLUOROQUINOLONESRESISTANCE INESCHERICHIA COLI OF URINARY TRACT INFECTIONS. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2017;7(25):91-100. Available from: https://sid.ir/paper/204598/en

IEEE: Copy

ELHAM SIASI, FARZANEH HOSSIENI, and POOYEH RAHIMINIA, “RELATION OFGYRA AND PARC GENES MUTATIONS WITH FLUOROQUINOLONESRESISTANCE INESCHERICHIA COLI OF URINARY TRACT INFECTIONS,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 7, no. 25, pp. 91–100, 2017, [Online]. Available: https://sid.ir/paper/204598/en

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