COMPARISON AND EVALUATION OF LABORATORY DIAGNOSTIC METHOD-SMEAR, CULTURE, MOLECULAR-IN THE FUNGAL INFECTION (ASPERGILLUS, DERMATOPHYTE AND MUCOR)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SHEIKH NASRIN; ZAKER BOSTANABAD SAEED; MIRZA AHMADI SINA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2017 | Volume:7 | Issue:25 Page(s): 19-26

Download Full-Text

Persian Verion

View:

741

Download:

Cites:

Information Journal Paper

Title

COMPARISON AND EVALUATION OF LABORATORY DIAGNOSTIC METHOD-SMEAR, CULTURE, MOLECULAR-IN THE FUNGAL INFECTION (ASPERGILLUS, DERMATOPHYTE AND MUCOR)

Author(s)

SHEIKH NASRIN | ZAKER BOSTANABAD SAEED | MIRZA AHMADI SINA | Issue Writer Certificate

Keywords

MUCORQ2

ASPERGILLUSQ2

DERMATOPHYTEQ3

SMEARQ1

CULTUREQ2

PCRQ2

Abstract

Aim and Background: Alarming increase in fungal infections, especially in immunocompromised patients against the infectious agents and the vast advances in diagnostic methods of such diseases, causing extensive investigations for the diagnosis of fungal infections with more modern methods in different groups of patients. Our main objective is to evaluate and compare methods of laboratory diagnosis of SMEAR-culture-molecular in fungal infections (dermatophytes, ASPERGILLUS, MUCOR) in clinical samples.Materials and Methods: since August 2014 to January 2015, 420 cases of suspected fungal infections, from the Razi Hospital, Baqiyatallah hospital and Massoud laboratory were collected. SMEAR preparation of the samples was carried out and on the basis of these findings, type of lesion and diagnosed by specialists, samples are grouped. CULTURE and molecular methods (PCR and PCRSequencing) at the same time was performed.Results: Of the 420 samples analyzed by direct SMEAR, 15 (5.3%) of ASPERGILLUS, 64 samples (15.2%) of DERMATOPHYTEs and zero sample of MUCOR were identified. In 15 specimens of ASPERGILLUS, 12 samples (80%) consisted with positive CULTUREs and 3 cases (20%) were negative and based on Nested PCR results, all of 15 samples were positive. Of 64 samples of DERMATOPHYTEs, 40 cases (62.5%) were positive by CULTURE method and 16 cases (25%) were negative.6 samples (3.9%) consisted with saprophytic contamination and 2 samples (12.3%) showed ASPERGILLUS growth at the injection site, respectively. Based on Nested PCR method, 62 samples (8.96%) showed positive result.Conclusion: The results of this study indicate that the PCR, with respect to its high sensitivity, bearings good capabilities for detection of fungal disease in the early stages of infection. Since the rapid and specific diagnosis of fungal infections, especially with regard to the genera and species of the fungal organisms, provide the possibility of proper and adequate applying of antifungal therapy protocols. Thus in this context, appropriate performance of rapid, specific and sensetive techniques would be necessary and inevitable.

Cites

No record.

References

Cite

APA: Copy

SHEIKH, NASRIN, ZAKER BOSTANABAD, SAEED, & MIRZA AHMADI, SINA. (2017). COMPARISON AND EVALUATION OF LABORATORY DIAGNOSTIC METHOD-SMEAR, CULTURE, MOLECULAR-IN THE FUNGAL INFECTION (ASPERGILLUS, DERMATOPHYTE AND MUCOR). NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 7(25), 19-26. SID. https://sid.ir/paper/204600/en

Vancouver: Copy

SHEIKH NASRIN, ZAKER BOSTANABAD SAEED, MIRZA AHMADI SINA. COMPARISON AND EVALUATION OF LABORATORY DIAGNOSTIC METHOD-SMEAR, CULTURE, MOLECULAR-IN THE FUNGAL INFECTION (ASPERGILLUS, DERMATOPHYTE AND MUCOR). NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2017;7(25):19-26. Available from: https://sid.ir/paper/204600/en

IEEE: Copy

NASRIN SHEIKH, SAEED ZAKER BOSTANABAD, and SINA MIRZA AHMADI, “COMPARISON AND EVALUATION OF LABORATORY DIAGNOSTIC METHOD-SMEAR, CULTURE, MOLECULAR-IN THE FUNGAL INFECTION (ASPERGILLUS, DERMATOPHYTE AND MUCOR),” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 7, no. 25, pp. 19–26, 2017, [Online]. Available: https://sid.ir/paper/204600/en

Related Journal Papers